Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)
Catalog Number: 27-0454
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 104
Isotype: Mouse IgG2a, κ

Staining of BALB/c (left) and SJL (right) splenocytes with 0.5 μg of APC-Alexa Fluor® 750 anti-mouse CD45.2 (104). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0454	Affinity Purified anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	N/A	N/A	FC IH/F IP
13-0454	Biotin anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	N/A	N/A	FC IH/F
11-0454	FITC anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	488	518	FC
12-0454	PE anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	488	575	FC
17-0454	Allophycocyanin (APC) anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	633	660	FC
35-0454	PE-Cy5.5 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	488	690	FC
56-0454	Alexa Fluor® 700 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (APC-Cy5.5 Equivalent)	633	723	FC
45-0454	PerCP-Cy5.5 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (PE-Cy5.5 replacement)	488	690	FC
47-0454	Allophycocyanin-eFluor^TM 780 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (APC-Alexa Fluor® 750 replacement)	633	780	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 104 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This 104 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Shen, F-W. (1981). "Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. In Monoclonal antibodies and T cell Hybridomas; Perspective and technical advances, eds. Hammerling, G.J., U. Hammerling, and J.F. Kearney": 25-31.

Related Products

Cat. 27-4724 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG2a, K Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.