Contents: Allophycocyanin-Alexa FluorŽ 750 (APC-Alexa FluorŽ 750) anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)
Catalog Number: 27-0454
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 104
Isotype: Mouse IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of BALB/c (left) and SJL (right) splenocytes with 0.5 μg of APC-Alexa FluorŽ 750 anti-mouse CD45.2 (104). Cells in the lymphocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-0454 |
FITC anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
488 |
518 |
FC
|
| 12-0454 |
PE anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
488 |
575 |
FC
|
| 13-0454 |
Biotin anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
N/A |
N/A |
FC
IH/F
|
| 14-0454 |
Affinity Purified anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
N/A |
N/A |
FC
IH/F
IP
|
| 17-0454 |
Allophycocyanin (APC) anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
633 |
660 |
FC
|
| 27-0454 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 35-0454 |
PE-Cy5.5 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
488 |
690 |
FC
|
| 45-0454 |
PerCP-Cy5.5 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
488 |
690 |
FC
|
| 56-0454 |
Alexa Fluor® 700 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 104 antibody has been reported for use in flow cytometric analysis.
The photo-emission properties and compensation required for Alexa FluorŽ 750 are similar to APC-Cy7; however Alexa FluorŽ 750 has several advantages when compared to APC-Cy7. The Alexa FluorŽ 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa FluorŽ 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa FluorŽ 750 as this may lead to degradation of the fluorochrome and loss of staining.
Applications Tested
This 104 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Shen, F-W. (1981). "Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. In Monoclonal antibodies and T cell Hybridomas; Perspective and technical advances, eds. Hammerling, G.J., U. Hammerling, and J.F. Kearney": 25-31.
Related Products
Cat.
27-4724 Allophycocyanin-Alexa FluorŽ 750 (APC-Alexa FluorŽ 750) Mouse IgG2a, K Isotype Control (APC-Cy7 Equivalent)
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Mouse Cellular Antigens Chart