Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Cy7 Equivalent)
Catalog Number: 27-0451
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 30-F11
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 bone marrow cells with PerCP-Cy5.5 anti-mouse Erythroid cells (TER-119) (cat. 45-5921) and 0.125 μg of APC-Alexa Fluor® 750 Rat IgG2b Isotype Control (cat. 27-4031) (left) or 0.125 μg of APC-Alexa Fluor® 750 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0451	Affinity Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FA FC IHC IP
16-0451	Functional Grade* Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FA FC IHC IP
13-0451	Biotin anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FC
11-0451	FITC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	518	FC
12-0451	PE anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	575	FC
15-0451	PE-Cy5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	670	FC
17-0451	APC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	633	660	FC
25-0451	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	760	FC
35-0451	PE-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	690	FC
56-0451	Alexa Fluor® 700 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Cy5.5 Equivalent)	633	723	FC
45-0451	PerCP-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (PE-Cy5.5 replacement)	488	690	FC
93-0451	eFluor^TM 605^NC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	350-500	605	FC
47-0451	Allophycocyanin-eFluor^TM 780 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Alexa Fluor® 750 Replacement)	633	780	FC
48-0451	eFluor^TM 450 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 30-F11 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.

Related Products

Cat. 27-0459 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD45 (LCA) (APC-Cy7 Equivalent) (clone HI30)
Cat. 27-4031 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2b Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.