Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse/human CD44 (Pgp-1, Ly-24)
Catalog Number: 27-0441
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃, 0.2% BSA, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation.
Protect this vial from light during storage, handling
& experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: IM7
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 splenocytes with PE anti-mouse CD3e (145-2C11) (cat. 12-0031) and staining buffer (autofluorescence) (left) or 0.125 μg of APC-Alexa Fluor® 750 anti-mo/hu CD44 (IM7) (right in FL4-H). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0441	Affinity Purified anti-mouse/human CD44 (Pgp-1, Ly-24)	N/A	N/A	FA FC IHC IP WB
16-0441	Functional Grade* Purified anti-mouse/human CD44 (Pgp-1, Ly-24)	N/A	N/A	FA FC
13-0441	Biotin anti-mouse/human CD44 (Pgp-1, Ly-24)	N/A	N/A	FC
11-0441	FITC anti-mouse/human CD44 (Pgp-1, Ly-24)	488	518	FC
12-0441	PE anti-mouse/human CD44 (Pgp-1, Ly-24)	488	575	FC
15-0441	PE-Cy5 anti-mouse/human CD44 (Pgp-1, Ly-24)	488	670	FC
17-0441	APC anti-mouse/human CD44 (Pgp-1, Ly-24)	633	660	FC
25-0441	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse/human CD44 (Pgp-1, Ly-24)	488	760	FC
35-0441	PE-Cy5.5 anti-mouse/human CD44 (Pgp-1, Ly-24)	488	690	FC
56-0441	Alexa Fluor® 700 anti-mouse/human CD44 (Pgp-1, Ly-24) (APC-Cy5.5 Equivalent)	633	723	FC
45-0441	PerCP-Cy5.5 anti-mouse/human CD44 (Pgp-1, Ly-24) (PE-Cy5.5 replacement)	488	690	FC
93-0441	eFluor^TM 605^NC anti-mouse/human CD44 (Pgp-1, Ly-24)	350-500	605	FC
47-0441	Allophycocyanin-eFluor^TM 780 anti-mouse/human CD44 (Pgp-1, Ly-24) (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0441	eFluor^TM 450 anti-mouse/human CD44 (Pgp-1, Ly-24) (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This IM7 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Trowbridge, I. S., J. Lesley, et al. 1982.Biochemical characterization and cellular distribution of a polymorphic, murine cell-surface glycoprotein expressed on lymphoid tissues. Immunogenetics 15(3): 299-312.
Lesley, J. and I. S. Trowbridge 1982. Genetic characterization of a polymorphic murine cell-surface glycoprotein. Immunogenetics 15(3): 313-20.
Maiti A, Maki G, Johnson P. TNF-alpha induction of CD44-mediated leukocyte adhesion by sulfation. Science. 1998. Oct 30;282(5390):941-3.

Related Products

Cat. 27-4031 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2b Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.