Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD27
Catalog Number: 27-0279
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation.
Protect this vial from light during storage, handling
& experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: O323
Isotype: Mouse IgG1, κ
HLDA No.: IV T-186

Staining of normal human peripheral blood cells with APC anti-human CD19 (HIB19) (cat. 17-0199) and staining buffer (autofluorescence) (left) or APC-Alexa Fluor® 750 anti-human CD27 (O323) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0279	Affinity Purified anti-human CD27	N/A	N/A	FC
13-0279	Biotin anti-human CD27	N/A	N/A	FC
11-0279	FITC anti-human CD27	488	518	FC
12-0279	PE anti-human CD27	488	575	FC
15-0279	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD27	488	670	FC
17-0279	APC anti-human CD27	633	660	FC
25-0279	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD27	488	760	FC
56-0279	Alexa Fluor® 700 anti-human CD27 (APC-Cy5.5 Equivalent)	633	723	FC
95-0279	eFluor^TM 650^NC anti-human CD27	350-500	650	FC
47-0279	Allophycocyanin-eFluor^TM 780 anti-human CD27 (APC-Alexa Fluor® 750 replacement)	633	780	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This O323 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This O323 antibody is offered in 2 formats:

- μg size: has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume). The pre-titrated test size contains 0.2% BSA.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-0271  FITC anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 12-0271  PE anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 13-0271  Biotin anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 14-0271  Affinity Purified anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 16-0271  Functional Grade Purified anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 17-0271  APC anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 27-4714  Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG1, K Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.