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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. Applications ReportedFor research use only, not for diagnostic or therapeutic use. This PC61.5 antibody has been reported for use in flow cytometric analysis. The photo-emission properties and compensation required for Alexa FluorŽ 750 are similar to APC-Cy7; however Alexa FluorŽ 750 has several advantages when compared to APC-Cy7. The Alexa FluorŽ 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa FluorŽ 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa FluorŽ 750 as this may lead to degradation of the fluorochrome and loss of staining. Applications TestedThis PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions and ConA-activated splenocyte culture. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. References
Lowenthal, J. W., P. Corthesy, et al. (1985)."High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." J Immunol 135(6): 3988-94. Lowenthal, J. W., C. Tougne, et al. (1985). "Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone." J Immunol 134(2): 931-9. Lowenthal, J. W., R. H. Zubler, et al. (1985)."Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." Nature 315(6021): 669-72. Hayashi, T, Hasegawa, K, Adachi, C. (2005) Elimination of CD4+CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZB × NZW F1 mice. Int J Exp Path 86: 289-296 (PC61.5, in vivo depletion, PubMed) Related ProductsCat. 12-0041 PE anti-mouse CD4 (L3T4) (clone GK1.5) Cat. 27-4301 Allophycocyanin-Alexa FluorŽ 750 (APC-Alexa FluorŽ 750) Rat IgG1 Isotype Control (APC-Cy7 Equivalent) Cat. 11-5773 FITC anti-mouse/rat Foxp3 (clone FJK-16s) Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc. |
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Copyright Š 2000-2008 eBioscience, Inc. Product For Research Use Only: Not for further distribution without written consent. |
Mouse Cellular Antigens Chart
