human CD20

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Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD20
Catalog Number: 27-0209
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 2H7
Isotype: Mouse IgG2b, κ
HLDA No.: IV B201

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
27-0209-71	25 tests	$145
27-0209-73	100 tests	$295

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with APC anti-human CD19 (HIB19) (cat. 17-0199) and APC-Alexa Fluor® 750 Mouse IgG2b Iso Cntrl (cat. 27-4732) (left) or APC-Alexa Fluor® 750 anti-human CD20 (2H7) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0209	FITC anti-human CD20	488	518	FC
12-0209	PE anti-human CD20	488	575	FC
13-0209	Biotin anti-human CD20	N/A	N/A	FC IH/F
14-0209	Affinity Purified anti-human CD20	N/A	N/A	FC IH/F
15-0209	PE-Cy5 anti-human CD20	488	670	FC
17-0209	Allophycocyanin (APC) anti-human CD20	633	660	FC
25-0209	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD20	488	760	FC
27-0209	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD20 (APC-Cy7 Equivalent)	633	779	FC
45-0209	PerCP-Cy5.5 anti-human CD20	488	690	FC
56-0209	Alexa Fluor® 700 anti-human CD20 (APC-Cy5.5 Equivalent)	633	723	FC
57-0209	Pacific Blue® anti-human CD20	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 2H7 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 20 μl (0.25 μg) per 100 μl blood.

References

Reinherz, E.L., et al. eds. 1985. Leukocyte Typing II (Vol. I, II, and III). Human Leukocyte Differentiation Antigens detected by Monoclonal Antibodies.
McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 27-4732 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG2b Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.