Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD8 (CD8a; alpha chain)
Catalog Number: 27-0088
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: RPA-T8
Isotype: Mouse IgG1, κ
HLDA No.: IV T171

Staining of normal human peripheral blood cells with APC anti-human CD19 (HIB19) (cat. 17-0199) and staining buffer (autofluorescence) (left) or APC-Alexa Fluor® 750 anti-human CD8a (RPA-T8) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0088	Affinity Purified anti-human CD8 (CD8a; alpha chain)	N/A	N/A	FC IH/F
11-0088	FITC anti-human CD8 (CD8a; alpha chain)	488	518	FC
12-0088	PE anti-human CD8 (CD8a; alpha chain)	488	575	FC
15-0088	PE-Cy5 anti-human CD8 (CD8a; alpha chain)	488	670	FC
17-0088	APC anti-human CD8 (CD8a; alpha chain)	633	660	FC
25-0088	PE-Cy7 anti-human CD8 (CD8a; alpha chain)	488	760	FC
27-0088	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD8 (CD8a; alpha chain) (APC-Cy7 Equivalent)	633	779	FC
45-0088	PerCP-Cy5.5 anti-human CD8 (CD8a; alpha chain)	488	690	FC
94-0088	eFluor^TM 625^NC anti-human CD8 (CD8a; alpha chain)	350-500	625	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RPA-T8 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.

Related Products

Cat. 11-0089    FITC anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 12-0089    PE anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 13-0089    Biotin anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 14-0089    Affinity Purified anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 16-0089    Functional Grade Purified anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 27-4714    Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG1, K Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.