mouse CD4 (L3T4)

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Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD4 (L3T4)
Catalog Number: 27-0042
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃, 0.2% BSA, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: RM4-5
Isotype: Rat IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
27-0042-81	50 ug	$120
27-0042-82	100 ug	$195

*International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with APC anti-mouse CD3e (145-2C11) (cat. 17-0031) and 0.125 μg of APC-Alexa Fluor® 750 Rat IgG2a Iso Cntrl (cat. 27-4321) (left) or 0.125 μg of APC-Alexa Fluor® 750 anti-mouse CD4 (RM4-5) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0042	FITC anti-mouse CD4 (L3T4)	488	518	FC
12-0042	PE anti-mouse CD4 (L3T4)	488	575	FC
13-0042	Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC
14-0042	Affinity Purified anti-mouse CD4 (L3T4)	N/A	N/A	FA FC IH/F
15-0042	PE-Cy5 anti-mouse CD4 (L3T4)	488	670	FC
16-0042	Functional Grade* Purified anti-mouse CD4 (L3T4)	N/A	N/A	FC
17-0042	APC anti-mouse CD4 (L3T4)	633	660	FC
25-0042	PE-Cy7 anti-mouse CD4 (L3T4)	488	760	FC
27-0042	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD4 (L3T4) (APC-Cy7 Equivalent)	633	779	FC
30-0042	Allophycocyanin-Cy5.5 (APC-Cy5.5) anti-mouse CD4 (L3T4) (Alexa Fluor® 700 Equivalent)	633	690	FC
35-0042	PE-Cy5.5 anti-mouse CD4 (L3T4)	488	690	FC
45-0042	PerCP-Cy5.5 anti-mouse CD4 (L3T4)	488	690	FC
56-0042	Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent)	633	723	FC
57-0042	Pacific Blue® anti-mouse CD4 (L3T4)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RM4-5 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This RM4-5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Okumura, K. 2000. Personal communication.
Wilde, D. B., P. Marrack, et al. 1983. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen- specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol 131(5): 2178-83.

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only.

Related Products

Cat. 11-0041    FITC anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 12-0041    PE anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 13-0041    Biotin anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 14-0041    Affinity Purified anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 15-0041    PE-Cy5 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 16-0041    Functional Grade Purified anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 17-0041    APC anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 19-0041    Cy5 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 25-0041    PE-Cy7 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 36-0041    Functional Grade Biotin anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 51-0041    Alexa Fluor® 647 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 53-0041    Alexa Fluor® 488 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 56-0041    Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent) (clone GK1.5)
Cat. 11-0043    Fluorescein isothiocyanate (FITC) anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 12-0043    Phycoerythrin (PE) anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 13-0043    Biotin anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 14-0043    Affinity Purified anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 27-4321    Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2a Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.