Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD3
Catalog Number: 27-0038
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: UCHT1
Isotype: Mouse IgG1, κ
HLDA No.: III 471

Staining of normal human peripheral blood cells with APC anti-human CD19 (HIB19) (cat. 17-0199) and staining buffer (autofluorescence) (left) or APC-Alexa Fluor® 750 anti-human CD3 (UCHT1) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0038	FITC anti-human CD3	488	518	FC
12-0038	PE anti-human CD3	488	575	FC
15-0038	PE-Cy5 anti-human CD3	488	670	FC
17-0038	APC anti-human CD3	633	660	FC
25-0038	PE-Cy7 anti-human CD3	488	760	FC
56-0038	Alexa Fluor® 700 anti-human CD3 (APC-Cy5.5 Equivalent)	633	723	FC
57-0038	Pacific Blue® anti-human CD3	402	421	FC
27-0038	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD3 (APC-Cy7 Equivalent)	633	779	FC
14-0038	Affinity Purified anti-human CD3	N/A	N/A	FA FC IH/F
16-0038	Functional Grade* Purified anti-human CD3	N/A	N/A	FA FC
13-0038	Biotin anti-human CD3	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This UCHT1 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-0037    FITC anti-human CD3 (clone OKT3)
Cat. 12-0037    PE anti-human CD3 (clone OKT3)
Cat. 14-0037    Affinity Purified anti-human CD3 (clone OKT3)
Cat. 16-0037    Functional Grade Purified anti-human CD3 (clone OKT3)
Cat. 51-0037    Alexa Fluor® 647 anti-human CD3 (clone OKT3)
Cat. 53-0037    Alexa Fluor® 488 anti-human CD3 (clone OKT3)
Cat. 57-0037    Pacific Blue® anti-human CD3 (clone OKT3)
Cat. 11-0039    FITC anti-human CD3 (clone HIT3a)
Cat. 12-0039    PE anti-human CD3 (clone HIT3a)
Cat. 14-0039    Affinity Purified anti-human CD3 (clone HIT3a)
Cat. 16-0039    Functional Grade Purified anti-human CD3 (clone HIT3a)
Cat. 27-4714    Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG1, K Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.