Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD3
Catalog Number: 27-0032
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 17A2
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and staining buffer (autofluorescence) (left) or 0.5 μg of APC-Alexa Fluor® 750 anti-mouse CD3 (17A2) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
51-0032	Alexa Fluor® 647 anti-mouse CD3 (CD3e, epsilon subunit)	633	668	FC
56-0032	Alexa Fluor® 700 anti-mouse CD3 (CD3e, epsilon subunit) (APC-Cy5.5 Equivalent)	633	723	FC
57-0032	Pacific Blue® anti-mouse CD3 (CD3e, epsilon subunit)	402	421	FC
27-0032	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD3 (CD3e, epsilon subunit) (APC-Cy7 Equivalent)	633	779	FC
14-0032	Affinity Purified Rat anti-mouse CD3 (CD3e, epsilon subunit)	N/A	N/A	FA FC IH/F
16-0032	Functional Grade* Purified Rat anti-mouse CD3 (CD3e, epsilon subunit)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits γ, δ and ε are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 17A2 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This 17A2 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Miescher, G. C., M. Schreyer, et al. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989: 23(2): 113-8.

Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC. Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol. 2002 Mar 15;168(6):3088-98.(17A2, IH/F, PubMed)

Related Products

Cat. 27-4031 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2b Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.