Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human Interferon-γ (IFN-γ)
Catalog Number: 25-7319
Sizes: 25 tests, 100 ug
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 4S.B3
Isotype: Mouse IgG1, κ

Intracellular staining of 5-hour PMA and Ionomycin-stimulated normal human peripheral blood mononuclear cells with PE-Cy7 Mouse IgG1, K Iso Cntrl (cat. 25-4714) (left) or PE-Cy7 anti-human IFNg (4S.B3) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7319	FITC anti-human IFNg (Interferon-gamma, IFN-g)	488	518	IC Flow
12-7319	PE anti-human IFNg (Interferon-gamma, IFN-g)	488	575	IC Flow
17-7319	APC anti-human IFNg (Interferon-gamma, IFN-g)	633	660	IC Flow
25-7319	Phycoerythrin-Cy7 (PE-Cy7) anti-human IFNg (Interferon-gamma, IFN-g)	488	760	IC Flow
51-7319	Alexa Fluor® 647 anti-human IFNg (Interferon-gamma, IFN-g)	633	668	IC Flow
53-7319	Alexa Fluor® 488 anti-human IFNg (Interferon-gamma, IFN-g)	488	519	IC Flow
57-7319	Pacific Blue® anti-human IFNg (Interferon-gamma, IFN-g)	402	421	IC Flow
45-7319	PerCP-Cy5.5 anti-human IFNg (Interferon-gamma, IFN-g)	488	690	IC Flow
14-7319	Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	IC Flow
13-7319	Biotin anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA det ELISPOT

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 4S.B3 monoclonal antibody reacts with interferon-γ (IFN-γ). The 4S.B3 antibody is a neutralizing antibody. Human IFN-γ is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ in synergy with other cytokines, such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 4S.B3 antibody has been reported for use in intracellular flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This 4S.B3 antibody is offered in 2 formats:

- μg size: has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by intracellular flow cytometric analysis. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume. The pre-titrated test size contains BSA and/or gelatin for protein stabilization.

References

Meager, A., S. Parti, et al. (1984). "Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon." J Interferon Res 4(4): 619-25.

Related Products

Cat. 25-4714    Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control
Cat. 00-8222    eBioscience IC Fixation Buffer
Cat. 14-8319    Recombinant Human IFNg (Interferon-gamma, IFN-g)
Cat. 00-8333    eBioscience Permeabilization Buffer(10X)
Cat. 88-8823    eBioscience Fixation & Permeabilization

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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