PE-Cy7 anti-mouse IFNg (Interferon-gamma, IFN-g) antibody: Cy7PE conjugate

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IFN-γ
Catalog Number: 25-7311
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7.
Clone: XMG1.2
Isotype: Rat IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
25-7311-71	25 tests	$125
25-7311-82	100 ug	$295

*International customers: Please contact your distributor for region specific pricing.

Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. The cells were surface-stained with APC anti-mouse CD4 (GK1.5) (cat. 17-0041) and intracellularly stained with PE-Cy7 rat IgG1 isotype control (cat. 25-4301)(left) or PE-Cy7 anti-mouse IFN-g (XMG1.2)(right).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7311	FITC anti-mouse IFNg (Interferon-gamma, IFN-g)	488	518	IC Flow
12-7311	PE anti-mouse IFNg (Interferon-gamma, IFN-g)	488	575	IC Flow
13-7311	Biotin anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA
14-7311	Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA cap
16-7311	Functional Grade* Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	NU
17-7311	APC anti-mouse IFNg (Interferon-gamma, IFN-g)	633	660	IC Flow
19-7311	Cy5 anti-mouse IFNg (Interferon-gamma, IFN-g)	633	670	IC Flow
25-7311	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IFNg (Interferon-gamma, IFN-g)	488	760	IC Flow
45-7311	PerCP-Cy5.5 anti-mouse IFNg (Interferon-gamma, IFN-g)	488	690	IC Flow
51-7311	Alexa Fluor® 647 anti-mouse IFNg (Interferon-gamma, IFN-g)	633	668	IC Flow
53-7311	Alexa Fluor® 488 anti-mouse IFNg (Interferon-gamma, IFN-g)	488	519	IC Flow
57-7311	Pacific Blue® anti-mouse IFNg (Interferon-gamma, IFN-g)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The XMG1.2 antibody reacts with mouse interferon-γ. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN-γ is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This XMG1.2 antibody is offered in 2 formats:

- μg size: has been tested by intracellular staining and flow cytometric analysis of ConA-stimulated mouse splencoytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of ConA-stimulated mouse splencoytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume). The pre-titrated test size contains BSA and/or gelatin for protein stabilization.

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.

Related Products

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Cat. 88-7234    Mouse IL-23 (p19/p40, IL23) ELISA Ready-SET-Go!
Cat. 00-8222    eBioscience IC Fixation Buffer
Cat. 14-8311    Recombinant Mouse IFNg (Interferon-gamma, IFN-g)
Cat. 00-8333    eBioscience Permeabilization Buffer(10X)
Cat. 88-8823    eBioscience Fixation & Permeabilization

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