Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human Interleukin-4 (IL-4)
Catalog Number: 25-7049
Sizes: 25 tests, 100 ug
Formulation: Phosphate buffer saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information, see PE-Cy7
Clone: 8D4-8
Isotype: Mouse IgG1, κ

Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. Subsequently, the cells were surface stained with FITC anti-human CD4 (OKT4) and intracellularly stained with PE-Cy7 mouse IgG1 isotype control (left) or PE-Cy7 anti-human IL-4 (8D4-8) (right).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-7049	Phycoerythrin (PE) anti-human IL-4 (Interleukin-4, IL4)	488	575	IC Flow
14-7049	Affinity Purified anti-human IL-4 (Interleukin-4, IL4)	N/A	N/A	ELISA cap ELISPOT cap
17-7049	Allophycocyanin (APC) anti-human IL-4 (Interleukin-4, IL4)	633	660	IC Flow
25-7049	Phycoerythrin-Cy7 (PE-Cy7) anti-human IL-4 (Interleukin-4, IL4)	488	760	IC Flow
51-7049	Alexa Fluor® 647 anti-human IL-4 (Interleukin-4, IL4)	633	668	IC Flow
53-7049	Alexa Fluor® 488 anti-human IL-4 (Interleukin-4, IL4)	488	519	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 8D4-8 antibody has been reported for use as a capture antibody for human IL-4 ELISA and for intracellular staining for flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

The fluorochrome-conjugated 8D4-8 antibody is offered in 2 formats:
- μg size: has been tested by intracellular staining for flow cytometric analysis. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- test size: has been pre-titrated and tested by intracellular staining for flow cytometric analysis. This can be used at 20 μl (0.125μg) per million cells in a 100 μl total staining volume.

References

Bird, C., et al. 1991. Development of immunoassays for human interleukin-3 and interleukin-4, some of which discriminate between different recombinant cDNA-derived molecules. Cytokine. 3: 562-567.

Related Products

Cat. 25-4714    Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control
Cat. 12-7048    PE anti-human IL-4 (Interleukin-4, IL4) (clone MP4-25D2)
Cat. 16-7048    Functional Grade Purified anti-human IL-4 (Interleukin-4, IL4) (clone MP4-25D2)
Cat. 17-7048    APC anti-human IL-4 (Interleukin-4, IL4) (clone MP4-25D2)
Cat. 14-8049    Recombinant Human IL-4 (Interleukin-4, IL4)
Cat. 00-8222    eBioscience IC Fixation Buffer
Cat. 00-8333    eBioscience Permeabilization Buffer(10X)
Cat. 88-8823    eBioscience Fixation & Permeabilization

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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