Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IL-4 (Interleukin-4)
Catalog Number: 25-7042
Sizes: 25 tests, 100 tests, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7.
Clone: BVD6-24G2
Isotype: Rat IgG1, κ

Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. The cells were surface stained with PE anti-mouse CD4 (GK1.5) and intracellularly stained with FITC anti-mouse IL-4 (BVD6-24G2).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7042	Affinity Purified anti-mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	IC Flow
13-7042	Biotin anti-mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	ELISA det ELISPOT
11-7042	FITC anti-mouse IL-4 (Interleukin-4, IL4)	488	518	IC Flow
25-7042	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IL-4 (Interleukin-4, IL4)	488	760	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This BVD6-24G2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This BVD6-24G2 antibody is offered in 2 formats:

- μg size: has been tested intracellular staining and flow cytometric analysis of restimulated mouse splenocyte cultures. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested intracellular staining and flow cytometric analysis of restimulated mouse splenocyte cultures. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume. The pre-titrated test size contains BSA and/or gelatin for protein stabilization.

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.
Sander, B., I. Hoiden, et al. (1993). "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." J Immunol Methods 166(2): 201-14.

Related Products

Cat. 25-4301    Phycoerythrin-Cy7 (PE-Cy7) Rat IgG1 Isotype Control
Cat. 11-7041    FITC anti-mouse IL-4 (Interleukin-4, IL4) (clone 11B11)
Cat. 12-7041    PE anti-mouse IL-4 (Interleukin-4, IL4) (clone 11B11)
Cat. 14-7041    Affinity Purified anti-mouse IL-4 (Interleukin-4, IL4) (clone 11B11)
Cat. 16-7041    Functional Grade Purified anti-mouse IL-4 (Interleukin-4, IL4) (clone 11B11)
Cat. 17-7041    APC anti-mouse IL-4 (Interleukin-4, IL4) (clone 11B11)
Cat. 25-7041    **PLEASE SEE CAT NO. 25-7042 INSTEAD** Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IL-4 (Interleukin-4, IL4) (clone 11B11)
Cat. 88-7944    Mouse IL-4 (Interleukin-4, IL4) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
Cat. 00-8222    eBioscience IC Fixation Buffer
Cat. 00-8333    eBioscience Permeabilization Buffer(10X)
Cat. 88-8823    eBioscience Fixation & Permeabilization

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

1.         Sale, lease, license or other transfer of the material or any material derived or produced from it.
2.         Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it
3.         Use of this material to perform services for a fee for third parties.

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