25-7041

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse Interleukin-4 (IL-4)
Catalog Number: 25-7041
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 11B11
Isotype: Rat IgG1, κ

Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. (Left) The cells were surface stained with APC anti-mouse CD4 (GK1.5) and intracellularly stained with PE anti-mouse IL-4 (11B11). (Right) The cells were surface stained with FITC anti-mouse CD4 and intracellularly stained with APC anti-mouse IL-4.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-7041	PE anti-mouse IL-4 (Interleukin-4, IL4)	488	575	IC Flow
14-7041	Affinity Purified anti-mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	ELISA cap ELISPOT cap
16-7041	Functional Grade* Purified anti-mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	NU
17-7041	APC anti-mouse IL-4 (Interleukin-4, IL4)	633	660	IC Flow
51-7041	Alexa Fluor® 647 anti-mouse IL-4 (Interleukin-4, IL4)	633	668	IC Flow
53-7041	Alexa Fluor® 488 anti-mouse IL-4 (Interleukin-4, IL4)	488	519	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 11B11 antibody has been reported for use in intracellular flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

This product is guaranteed for 6 months upon receipt when stored properly.

Applications Tested

The 11B11 antibody is offered in 2 formats:

- μg size: has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by intracellular flow cytometric analysis. This can be used at 20 μl per million cells in a 100 μl total staining volume. The pre-titrated test size contains 0.2% BSA.

References

Ohara, J. and W. E. Paul. 1985. Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1. Nature. 315: 333-336.
Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

Related Products

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