Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)
Catalog Number: 25-5971
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7.
Clone: DX5
Isotype: Rat IgM, κ

Staining of C57Bl/6 splenocytes with FITC anti-mouse NK-1.1 (PK136) (cat. 11-5941) and staining buffer (autofluorescence) (left) or 0.25 μg PE-Cy7 anti-mouse CD49b (DX5) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5971	FITC anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	488	518	FC
12-5971	PE anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	488	575	FC
13-5971	Biotin anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	N/A	N/A	FC
14-5971	Affinity Purified anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	N/A	N/A	FC
17-5971	APC anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	633	660	FC
25-5971	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	488	760	FC
36-5971	Functional Grade* Biotin anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer cells and a subset of T cells. DX5 reacts with all strains of mouse tested including the most commonly used strains, BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL and 129. Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5⁺PK136^- and DX5^-PK136⁺ cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This DX5 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This DX5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hussell, T. and P. J. Openshaw (1998). "Intracellular IFN-gamma expression in natural killer cells precedes lung CD8+ T cell recruitment during respiratory syncytial virus infection." J Gen Virol 79(Pt 11): 2593-601.
Vos, Q., J. R. Ortaldo, et al. (1998). "Phenotypic and functional characterization of a panel of cytotoxic murine NK cell clones that are heterogeneous in their enhancement of Ig secretion in vitro." Int Immunol 10(8): 1093-101.
Arase, H., T. Saito, et al. (2001). Cutting edge: the mouse NK cell-associated antigen recognized by DX5 moncoclonal antibody is CD49b (alpha(2) integrin, very late antigen-2). J Immunol 167(3): 1141-4.

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

1.         Sale, lease, license or other transfer of the material or any material derived or produced from it.
2.         Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it
3.         Use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO eBioscience AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.