Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse Ly-6G (Gr-1, Gr1)
Catalog Number: 25-5931
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7.
Clone: RB6-8C5
Isotype: Rat IgG2b, κ

Two-color surface staining of mouse bone marrow with anti-mouse Ly-6G (RB6-8C5) FITC and anti-mouse CD45R/B220 (RA3-6B2) PE. Total viable cells were used for analysis

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5931	FITC anti-mouse Ly-6G (Gr-1, Gr1)	488	518	FC
12-5931	PE anti-mouse Ly-6G (Gr-1, Gr1)	488	575	FC
13-5931	Biotin anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FC
14-5931	Affinity Purified anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FA FC IH/F IHC(Paraffin) IP WB
15-5931	PE-Cy5 anti-mouse Ly-6G (Gr-1, Gr1)	488	670	FC
16-5931	Functional Grade* Purified anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FA FC
17-5931	Allophycocyanin (APC) anti-mouse Ly-6G (Gr-1, Gr1)	633	660	FC
19-5931	Cy5 anti-mouse Ly-6G (Gr-1, Gr1)	633	670	FC
25-5931	PE-Cy7 anti-mouse Ly-6G (Gr-1, Gr1)	488	760	FC
27-5931	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse Ly-6G (Gr-1, Gr1) (APC-Cy7 Equivalent)	633	779	FC
35-5931	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse Ly-6G (Gr-1, Gr1)	488	690	FC
36-5931	Functional Grade* Biotin anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FC
45-5931	PerCP-Cy5.5 anti-mouse Ly-6G (Gr-1, Gr1)	488	690	FC
51-5931	Alexa Fluor® 647 anti-mouse Ly-6G (Gr-1, Gr1)	633	668	FC
53-5931	Alexa Fluor® 488 anti-mouse Ly-6G (Gr-1, Gr1)	488	519	FC
56-5931	Alexa Fluor® 700 anti-mouse Ly-6G (Gr-1, Gr1) (APC-Cy5.5 Equivalent)	633	723	FC
57-5931	Pacific Blue® anti-mouse Ly-6G (Gr-1, Gr1)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G and Ly-6C, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RB6-8C5 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sasmono RT, Ehrnsperger A, Cronau SL, Ravasi T, Kandane R, Hickey MJ, Cook AD, Himes SR, Hamilton JA, Hume DA. Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1. J Leukoc Biol. 2007 Jul;82(1):111-23. (RB6-8C5; Cross-reactivity to Ly-6G and Ly-6C)

Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res. 2007 May 1;67(9):4346-52. (RB6-8C5, in vivo depletion, PubMed)

Fan J, Li Y, Vodovotz Y, Billiar TR, Wilson MA. Hemorrhagic shock-activated neutrophils augment TLR4 signaling-induced TLR2 upregulation in alveolar macrophages: role in hemorrhage-primed lung inflammation. Am J Physiol Lung Cell Mol Physiol. 2006 Apr;290(4):L738-L746. (RB6-8C5, in vivo depletion, PubMed)

Alvarez MJ, Prada F, Salvatierra E, Bravo AI, Lutzky VP, Carbone C, Pitossi FJ, Chuluyan HE, Podhajcer OL. Secreted protein acidic and rich in cysteine produced by human melanoma cells modulates polymorphonuclear leukocyte recruitment and antitumor cytotoxic capacity. Cancer Res. 2005 Jun 15;65(12):5123-32. (RB6-8C5, IHC paraffin)

Brown CR, Blaho VA, Loiacono CM. Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2. J Immunol. 2003 Jul 15;171(2):893-901. (RB6-8C5, IHC paraffin)

Miura M, Fu X, Zhang QW, Remick DG, Fairchild RL. Neutralization of Gro alpha and macrophage inflammatory protein-2 attenuates renal ischemia/reperfusion injury. Am J Pathol. 2001 Dec;159(6):2137-45. (RB6-8C5, IHC frozen)

Dunussi-Joannopoulos K, Runyon K, Erickson J, Schaub RG, Hawley RG, Leonard JP. Vaccines with interleukin-12-transduced acute myeloid leukemia cells elicit very potent therapeutic and long-lasting protective immunity. Blood. 1999 Dec 15;94(12):4263-73. (RB6-8C5, IHC frozen)

Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993 Sep 1;151(5):2399-408. (RB6-8C5; Cross-reactivity to Ly-6G and Ly-6C)

Hestdal K, Ruscetti FW, Ihle JN, Jacobsen SE, Dubois CM, Kopp WC, Longo DL, Keller JR. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J Immunol. 1991 Jul 1;147(1):22-8.

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