PE-Cy7 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) antibody: Cy7PE conjugate

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)
Catalog Number: 25-5921
Formulation: Phosphate buffer saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7
Clone: TER-119
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
25-5921-81	50 ug	$120
25-5921-82	100 ug	$195

*International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 bone marrow cells with FITC anti-mouse CD45 (30-F11) (cat. 11-0451) and 0.25 μg of PE-Cy7 Rat IgG2b Iso Cntrl (cat. 25-4331) (left) or 0.25 μg of PE-Cy7 anti-mouse Erythroid cells (TER-119) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5921	FITC anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	518	FC
12-5921	PE anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	575	FC
13-5921	Biotin anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	N/A	N/A	FC
14-5921	Affinity Purified anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	N/A	N/A	FC IHC IP WB
15-5921	PE-Cy5 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	670	FC
16-5921	Functional Grade* Purified anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	N/A	N/A	FC
17-5921	APC anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	633	660	FC
25-5921	PE-Cy7 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	760	FC
27-5921	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) (APC-Cy7 Equivalent)	633	779	FC
35-5921	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	690	FC
36-5921	Functional Grade* Biotin anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	N/A	N/A	FC
45-5921	PerCP-Cy5.5 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	690	FC
57-5921	Pacific Blue® anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The TER-119 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

The TER-119 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kina, T., K. Ikuta, et al. (2000). "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." Br J Haematol 109(2): 280-87.
Vannucchi, A. M., F. Paoletti, et al. (2000). "Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice." Blood 95(8): 2559-68.

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