Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IgM
Catalog Number: 25-5790
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: II/41
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 bone marrow cells with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.125 μg of PE-Cy7 Rat IgG2a Iso Cntrl (cat. 25-4321) (left) or 0.125 μg of PE-Cy7 II/41 (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5790	Affinity Purified anti-mouse IgM	N/A	N/A	ELISA FC IH/F
13-5790	Biotin anti-mouse IgM	N/A	N/A	FC
11-5790	Fluorescein (FITC) anti-mouse IgM	488	518	FC
12-5790	Phycoerythrin (PE) anti-mouse IgM	488	575	FC
15-5790	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse IgM	488	670	FC
17-5790	Allophycocyanin (APC) anti-mouse IgM	633	660	FC
25-5790	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IgM	488	760	FC
35-5790	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse IgM	488	690	FC
36-5790	Functional Grade* Biotin anti-mouse IgM	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This II/41 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. 1993. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 150(12):5252-62.

Related Products

Cat. 25-4321    Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2a Isotype Control
Cat. 11-5890    FITC anti-mouse IgM (clone eB121-15F9)
Cat. 12-5890    PE anti-mouse IgM (clone eB121-15F9)
Cat. 13-5890    Biotin anti-mouse IgM (clone eB121-15F9)
Cat. 14-5890    Affinity Purified anti-mouse IgM (clone eB121-15F9)

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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