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Product Information


Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IgM
Catalog Number: 25-5790
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: II/41
Host/Isotype: Rat IgG2a, κ
 
 
mouse IgM antibody, Phycoerythrin-Cy7 (PE-Cy7) conjugate
Staining of C57Bl/6 bone marrow cells with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.06 μg of PE-Cy7 Rat IgG2a Isotype Control (cat. 25-4321) (left) or 0.06 μg of PE-Cy7 anti-mouse IgM (II/41) (right). Cells in the lymphocyte gate were used for analysis. 

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
11-5790 Fluorescein (FITC) anti-mouse IgM 488 518 FC
12-5790 Phycoerythrin (PE) anti-mouse IgM 488 575 FC
13-5790 Biotin anti-mouse IgM N/A N/A FC
14-5790 Affinity Purified anti-mouse IgM N/A N/A ELISA FC IH/F
15-5790 Phycoerythrin-Cy5 (PE-Cy5) anti-mouse IgM 488 670 FC
17-5790 Allophycocyanin (APC) anti-mouse IgM 633 660 FC
25-5790 Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IgM 488 760 FC
35-5790 Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse IgM 488 690 FC
36-5790 Functional Grade* Biotin anti-mouse IgM N/A N/A FC
46-5790 PerCP-eFluor® 710 anti-mouse IgM 488 710 FC
95-5790 eFluor® 650NC anti-mouse IgM 350-500 650 FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description


The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.


Applications Reported


For research use only, not for diagnostic or therapeutic use. This II/41 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.


Applications Tested


The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.


References


Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. 1993. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 150(12):5252-62.


Related Products


Cat. 25-4321  Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2a Isotype Control
Cat. 11-5890  FITC anti-mouse IgM (clone eB121-15F9)
Cat. 12-5890  PE anti-mouse IgM (clone eB121-15F9)
Cat. 13-5890  Biotin anti-mouse IgM (clone eB121-15F9)
Cat. 14-5890  Affinity Purified anti-mouse IgM (clone eB121-15F9)

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.

COMMERCIAL USE shall include:
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