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Product Information

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Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IgM Catalog Number: 25-5790 Sizes: 50 ug, 100 ug Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly. Clone: II/41 Host/Isotype: Rat IgG2a, κ

Staining of C57Bl/6 bone marrow cells with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.06 μg of PE-Cy7 Rat IgG2a Isotype Control (cat. 25-4321) (left) or 0.06 μg of PE-Cy7 anti-mouse IgM (II/41) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5790	Fluorescein (FITC) anti-mouse IgM	488	518	FC
12-5790	Phycoerythrin (PE) anti-mouse IgM	488	575	FC
13-5790	Biotin anti-mouse IgM	N/A	N/A	FC
14-5790	Affinity Purified anti-mouse IgM	N/A	N/A	ELISA FC IH/F
15-5790	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse IgM	488	670	FC
17-5790	Allophycocyanin (APC) anti-mouse IgM	633	660	FC
25-5790	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IgM	488	760	FC
35-5790	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse IgM	488	690	FC
36-5790	Functional Grade* Biotin anti-mouse IgM	N/A	N/A	FC
46-5790	PerCP-eFluor® 710 anti-mouse IgM	488	710	FC
95-5790	eFluor® 650NC anti-mouse IgM	350-500	650	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. This II/41 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

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Applications Tested

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The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. 1993. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 150(12):5252-62.

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Related Products

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Cat. 25-4321  Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2a Isotype Control
Cat. 11-5890  FITC anti-mouse IgM (clone eB121-15F9)
Cat. 12-5890  PE anti-mouse IgM (clone eB121-15F9)
Cat. 13-5890  Biotin anti-mouse IgM (clone eB121-15F9)
Cat. 14-5890  Affinity Purified anti-mouse IgM (clone eB121-15F9)

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