Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse FR4 (Folate receptor 4, FBP, FRδ)
Catalog Number: 25-5445
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
Clone: eBio12A5
Isotype: Rat IgG2b, κ

Staining of BALB/c splenocytes with FITC anti-mouse CD4 (GK1.5) (cat. 11-0041) and 0.25 μg of PE-Cy7 Rat IgG2b Iso Cntrl (cat. 25-4331) (left) or 0.25 μg of PE-Cy7 anti-mouse FR4 (eBio12A5) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5445	Affinity Purified anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	N/A	N/A	FC IP
13-5445	Biotin anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	N/A	N/A	FC
11-5445	Fluorescein isothiocyanate (FITC) anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	488	518	FC
12-5445	Phycoerythrin (PE) anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	488	575	FC
25-5445	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	488	760	FC
51-5445	Alexa Fluor® 647 anti-mouse FR4 (Folate receptor 4, FBP, FRdelta, FRd)	633	668	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody eBio12A5 recognizes FR4, also known as Folate receptor 4, FR δ and FBP3 (folate binding protein3). FR4 is a heavily glycosylated 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate. Natural T regs have high levels of FR4 and together with CD25 can be used to distinguish natural Tregs, effector T cells, memory-like T cells and naïve T cells. FR4^hi CD25^hi cells are natural Tregs with high levels of Foxp3. FR4^hi CD25^lo cells are identified as central memory T cells which upon stimulation can proliferate and produce large amounts of IL-2. In contrast FR4^lo CD25^hi secrete proinflammatory cytokines such as IFNγ and IL-17 and have been characterized as effector memory T cells.

Based on co-staining studies, the epitopes recognized by eBioTH6 (cat 51-5446) and eBio12A5 are different thereby allowing functional studies with eBioTH6 to be evaluated with eBio12A5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio12A5 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This eBio12A5 antibody has been tested by flow cytyometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Elnakat H, Ratnam M. Distribution, functionality and gene regulation of folate receptor isoforms: implications in targeted therapy. Adv Drug Deliv Rev. 2004 Apr 29;56(8):1067-84.

Spiegelstein O, Eudy JD, Finnell RH. Identification of two putative novel folate receptor genes in humans and mouse. Gene. 2000 Nov 27;258(1-2):117-25.

Sugimoto N, Oida T, Hirota K, Nakamura K, Nomura T, Uchiyama T, Sakaguchi S. Foxp3-dependent and -independent molecules specific for CD25+CD4+ natural regulatory T cells revealed by DNA microarray analysis. Int Immunol. 2006 Aug;18(8):1197-209.

Yamaguchi T, Hirota K, Nagahama K, Ohkawa K, Takahashi T, Nomura T, Sakaguchi S. Control of immune response by antigen-specific regulatory T cells expressing the folate receptor. Immunity July 2007 ePub (eBio12A5 FC, IP)

Related Products

Cat. 11-0041    FITC anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 25-4031    Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2b Isotype Control
Cat. 00-5523    eBioscience Foxp3 Staining Buffer Set
Cat. 12-5773    PE anti-mouse/rat Foxp3 (clone FJK-16s)

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