Contents: Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, κ Isotype Control
Catalog Number: 25-4714
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Isotype: Mouse IgG1, κ
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-4714 |
Affinity Purified Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
IC Flow
IP
WB
|
| 16-4714 |
Functional Grade* Purified Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
|
| 13-4714 |
Biotin Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
|
| 11-4714 |
Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control |
488 |
518 |
FC
|
| 12-4714 |
Phycoerythrin (PE) Mouse IgG1, K Isotype Control |
488 |
575 |
FC
|
| 15-4714 |
Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control |
488 |
670 |
FC
|
| 17-4714 |
Allophycocyanin (APC) Mouse IgG1, K Isotype Control |
633 |
660 |
FC
|
| 25-4714 |
Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control |
488 |
760 |
FC
|
| 35-4714 |
Phycoerythrin-Cy5.5 (PE-Cy5.5) Mouse IgG1, K Isotype Control |
488 |
690 |
FC
|
| 51-4714 |
Alexa Fluor® 647 Mouse IgG1, K Isotype Control |
633 |
668 |
FC
|
| 53-4714 |
Alexa Fluor® 488 Mouse IgG1, K Isotype Control |
488 |
519 |
FC
|
| 56-4714 |
Alexa Fluor® 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-4714 |
Pacific Blue® Mouse IgG1, K Isotype Control |
402 |
421 |
FC
|
| 27-4714 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG1, K Isotype Control (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 45-4714 |
PerCP-Cy5.5 Mouse IgG1, K Isotype Control |
488 |
690 |
FC
|
| 93-4714 |
eFluorTM 605NC Mouse IgG1, Isotype Control |
350-500 |
605 |
FC
|
| 94-4714 |
eFluorTM 625NC Mouse IgG1 Isotype Control |
350-500 |
625 |
FC
|
| 95-4714 |
eFluorTM 650NC Mouse IgG1, K Isotype Control |
350-500 |
650 |
FC
|
| 47-4714 |
Allophycocyanin-eFluorTM 780 Mouse IgG1, K Isotype Control (APC-Alexa Fluor® 750 replacement) |
633 |
780 |
FC
|
| 48-4714 |
eFluorTM 450 Mouse IgG1, K Isotype Control (Pacific Blue® replacement) |
405 |
450 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.
Description
The monoclonal mouse IgG1, κ, immunoglobulin is useful as an isotype control.
Applications Reported
For research use only, not for diagnostic or therapeutic use. PE-Cy7 Mouse IgG1, K Iso Cntrl has been reported for use in flow cytometric analysis. This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.
Applications Tested
PE-Cy7 Mouse IgG1, K Iso Cntrl has been pre-titrated and tested by flow cytometric analysis of human peripheral leukocytes. Please refer to the antibody vial for the recommended test volume to use for one million cells in a 100 μl total staining volume.
Related Products
Cat. 25-4714 Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control
FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT
FOR THERAPEUTIC OR IN VIVO APLICATIONS.
OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER
U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.
THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC.
AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM
PHARMACIA BIOTECH INC. THIS PRODUCT
IS LICENSED FOR SALE ONLY FOR RESEARCH.
IT IS NOT LICENSED FOR ANY OTHER USE.
THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:
1.
Sale, lease, license or other transfer of the material or any material derived or produced from it.
2.
Sale, lease, license or other grant of rights to use this material or any other material derived
or produced from it
3.
Use of this material to perform services for a fee for third parties.
IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE,
RETURN THIS MATERIAL, UNOPENED TO eBioscience AND ANY MONEY PAID FOR THE
MATERIAL WILL BE REFUNDED.
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