Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)
Catalog Number: 25-1278
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7.
Clone: eBioRDR5
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with FITC anti-human CD3 (OKT3) (cat. 11-0037) and 0.5 μg of PE-Cy7 Mouse IgG1, K Iso Cntrl (cat. 25-4714) (left) or 0.5 μg of PE-Cy7 anti-human CD127 (eBioRDR5) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1278	Fluorescein isothiocyanate (FITC) anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	488	518	FC
12-1278	Phycoerythrin (PE) anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	488	575	FC
13-1278	Biotin anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	N/A	N/A	FC
14-1278	Affinity Purified anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	N/A	N/A	FC IH/F
15-1278	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	488	670	FC
16-1278	Functional Grade* Purified anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	N/A	N/A	FC
25-1278	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	488	760	FC
27-1278	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) (APC-Cy7 Equivalent)	633	779	FC
45-1278	PerCP-Cy5.5 anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	488	690	FC
51-1278	Alexa Fluor® 647 anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	633	668	FC
57-1278	Pacific Blue® anti-human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor α). CD127 complexes with CD132, also known as the common γ chain (γc), to form the multi-functional IL-7 receptor (IL-7R). CD127 is a type I glycoprotein with a molecular weight of 75-80 kDa and is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation. Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways including the Jak/STAT pathway. IL-7 is indispensible for the development of lymphocytes, and the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is know to be involved in the regulation of T cell receptor (TCR) locus rearrangement in γδ T cells.

Interestingly, recently it has been demonstrated that CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). While the co-expression of CD4 and CD25 has become widely used as an indicator of T regs, this method of identification may also include cells without suppressive activity. It has clearly been shown that CD4+CD25+ cells that have down-regulated the expression of CD127 are significantly more highly-enriched for the regulatory T population, as defined by expression of the T reg-specific transcription factor Foxp3 and the suppressive activity of these cells, in vitro.

Binding of the eBioRDR5 monoclonal antibody to PBMCs is blocked by pre-incubation of the cells with recombinant human IL-7 (cat. 14-8079).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioRDR5 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This eBioRDR5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Goodwin RG, Friend D, Ziegler SF, Jerzy R, Falk BA, Gimpel S, Cosman D, Dower SK, March CJ, Namen AE, et al. Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily. Cell. 1990 Mar 23;60(6):941-51.

Corcoran AE, Smart FM, Cowling RJ, Crompton T, Owen MJ, Venkitaraman AR. The interleukin-7 receptor alpha chain transmits distinct signals for proliferation and differentiation during B lymphopoiesis. EMBO J. 1996 Apr 15;15(8):1924-32.

Maraskovsky E, O'Reilly LA, Teepe M, Corcoran LM, Peschon JJ, Strasser A. Bcl-2 can rescue T lymphocyte development in interleukin-7 receptor-deficient mice but not in mutant rag-1-/- mice. Cell. 1997 Jun 27;89(7):1011-9.

Liu W, Putnam AL, Xu-Yu Z, Szot GL, Lee MR, Zhu S, Gottlieb PA, Kapranov P, Gingeras TR, de St Groth BF, Clayberger C, Soper DM, Ziegler SF, Bluestone JA. CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells. J Exp Med. 2006 Jul 10;203(7):1701-11.

Seddiki N, Santner-Nanan B, Martinson J, Zaunders J, Sasson S, Landay A, Solomon M, Selby W, Alexander SI, Nanan R, Kelleher A, Fazekas de St Groth B. Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J Exp Med. 2006 Jul 10;203(7):1693-700.

Related Products

Cat. 25-1271    PE-Cy7 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) (clone A7R34)
Cat. 25-4714    Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control

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