Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD90.2 (Thy-1.2, Thy1.2)
Catalog Number: 25-0902
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 53-2.1
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.03 μg of PE-Cy7 Rat IgG2a Iso Cntrl (cat. 25-4321) (left) or 0.03 μg of PE-Cy7 anti-mouse CD90.2 (53-2.1) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0902	Affinity Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FC IH/F IP
13-0902	Biotin anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FC
11-0902	FITC anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	518	FC
12-0902	PE anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	575	FC
15-0902	PE-Cy5 anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	670	FC
17-0902	APC anti-mouse CD90.2 (Thy-1.2, Thy1.2)	633	660	FC
25-0902	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	760	FC
95-0902	eFluor^TM 650^NC anti-mouse CD90.2 (Thy-1.2, Thy1.2)	350-500	650	FC
47-0902	Allophycocyanin-eFluor^TM 780 anti-mouse CD90.2 (Thy-1.2, Thy1.2) (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0902	eFluor^TM 450 anti-mouse CD90.2 (Thy-1.2, Thy1.2) (Pacific Blue® replacement)	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 53-2.1 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.

Related Products

Cat. 11-0903  FITC anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 30-H12)
Cat. 12-0903  PE anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 30-H12)
Cat. 13-0903  Biotin anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 30-H12)
Cat. 14-0903  Affinity Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 30-H12)
Cat. 16-0903  Functional Grade Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 30-H12)
Cat. 25-4321  Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2a Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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