Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD69 (Very Early Activation Antigen, VEA)
Catalog Number: 25-0691
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: H1.2F3
Isotype: Armenian Hamster IgG

Surface staining of ConA stimulated splenocytes with anti-mouse CD69 (H1.2F3) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0691	Affinity Purified anti-mouse CD69 (Very Early Activation Antigen, VEA)	N/A	N/A	FA FC IHC IP
16-0691	Functional Grade* Purified anti-mouse CD69 (Very Early Activation Antigen, VEA)	N/A	N/A	FA FC
13-0691	Biotin anti-mouse CD69 (Very Early Activation Antigen, VEA)	N/A	N/A	FC
11-0691	FITC anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	518	FC
12-0691	PE anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	575	FC
15-0691	PE-Cy5 anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	670	FC
17-0691	Allophycocyanin (APC) anti-mouse CD69 (Very Early Activation Antigen, VEA)	633	660	FC
25-0691	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD69 (Very Early Activation Antigen, VEA)	488	760	FC
45-0691	PerCP-Cy5.5 anti-mouse CD69 (Very Early Activation Antigen, VEA) (PE-Cy5.5 replacement)	488	690	FC
93-0691	eFluor^TM 605^NC anti-mouse CD69 (Very Early Activation Antigen, VEA)	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The H1.2F3 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

This product is guaranteed for 6 months upon receipt when stored properly.

Applications Tested

The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Yokoyama, W. M., F. Koning, et al. (1988). "Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation." J Immunol 141(2): 369-76.

Related Products

Cat. 25-4888  Phycoerythrin-Cy7 (PE-Cy7) Armenian Hamster IgG Isotype Control (clone eBio299Arm)

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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