Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human CD62L (L-selectin)
Catalog Number: 25-0629
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: DREG-56 (DREG56)
Isotype: Mouse IgG1, κ
HLDA No.: V S056

Staining of normal human lysed whole blood with staining buffer (autofluorescence) (open histogram) or PE-Cy7 anti-human CD62L (Dreg 56) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0629	FITC anti-human CD62L (L-selectin)	488	518	FC
12-0629	PE anti-human CD62L (L-selectin)	488	575	FC
14-0629	Affinity Purified anti-human CD62L (L-selectin)	N/A	N/A	FA FC IH/F WB
15-0629	PE-Cy5 anti-human CD62L (L-selectin)	488	670	FC
16-0629	Functional Grade* Purified anti-human CD62L (L-selectin)	N/A	N/A	FA FC
17-0629	Allophycocyanin (APC) anti-human CD62L (L-selectin)	633	660	FC
25-0629	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD62L (L-selectin)	488	760	FC
27-0629	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD62L (L-selectin) (APC-Cy7 Equivalent)	633	779	FC
93-0629	eFluor^TM 605^NC anti-human CD62L (L-selectin)	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This DREG-56 (DREG56) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (0.5 μg) per 1 million cells in 100 μl total staining volume.

References

Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 25-4714    Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control

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