Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)
Catalog Number: 25-0453
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: A20
Isotype: Mouse IgG2a, κ

Staining of BALB/c (left) and SJL (right) splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 μg PE-Cy7 anti-mouse CD45.1 (A20) (colored histogram). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0453	Affinity Purified anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	N/A	N/A	FC IP
13-0453	Biotin anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	N/A	N/A	FC
11-0453	FITC anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	488	518	FC
12-0453	PE anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	488	575	FC
15-0453	PE-Cy5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	488	670	FC
17-0453	APC anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	633	660	FC
19-0453	Cy5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	633	670	FC
25-0453	PE-Cy7 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	488	760	FC
35-0453	PE-Cy5.5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	488	690	FC
45-0453	PerCP-Cy5.5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) (PE-Cy5.5 replacement)	488	690	FC
93-0453	eFluor^TM 605^NC anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	350-500	605	FC
47-0453	Allophycocyanin-eFluor^TM 780 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0453	eFluor^TM 450 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) (Pacific Blue® replacement)	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The A20 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

The A20 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Shen, F-W. (1981). "Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. In Monoclonal antibodies and T cell Hybridomas; Perspective and technical advances, eds. Hammerling, G.J., U. Hammerling, and J.F. Kearney": 25-31.

Related Products

Cat. 25-4724  Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG2a, K Isotype Control

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