Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)
Catalog Number: 25-0451
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 30-F11
Isotype: Rat IgG2b, κ

Staining of BALB/c splenocytes with .06 μg of PE-Cy7 Rat IgG2b Isotype Control (cat. 25-4031) (open histogram) or .06 μg of PE-Cy7 anti-mouse CD45 (30-F11) (colored histogram). Total splenocytes were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0451	FITC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	518	FC
12-0451	PE anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	575	FC
15-0451	PE-Cy5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	670	FC
17-0451	APC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	633	660	FC
25-0451	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	760	FC
35-0451	PE-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	690	FC
56-0451	Alexa Fluor® 700 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Cy5.5 Equivalent)	633	723	FC
57-0451	Pacific Blue® anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	402	421	FC
27-0451	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Cy7 Equivalent)	633	779	FC
45-0451	PerCP-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	690	FC
93-0451	eFluor^TM 605^NC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	350-500	605	FC
14-0451	Affinity Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FA FC IHC IP
16-0451	Functional Grade* Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FA FC IHC IP
13-0451	Biotin anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 30-F11 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This 30-F11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.

Related Products

Cat. 25-0459    Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45 (LCA) (clone HI30)
Cat. 25-4031    Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2b Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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