Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human CD39
Catalog Number: 25-0399
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: eBioA1 (A1)
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with FITC anti-human CD19 (HIB19) (cat. 11-0199) and 0.25 μg of PE-Cy7 Mouse IgG1, K Iso Cntrl (cat. 25-4714) (left) or PE-Cy7 anti-human CD39 (A1) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0399	Affinity Purified anti-human CD39	N/A	N/A	FC IH/F
11-0399	Fluorescein isothiocyanate (FITC) anti-human CD39	488	518	FC
12-0399	Phycoerythrin (PE) anti-human CD39	488	575	FC
25-0399	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD39	488	760	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioA1 monoclonal antibody reacts with human CD39 also known as ENTPD1- ectonucleoside triphosphate diphosphohydrolase 1 or NTPDase. CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer. It is the most prominent ectoenzyme of the immune system. The function of CD39 is to effectively remove toxic extracellular ATP by converting it to ADP or AMP. CD39 is thought to work together with CD73 to hydrolyze ATP and has been well characterized on langerhan cells. Expression of CD39 is originally identified on activated lymphocytes. Expression is also found on a subset of T cells, B cells and dendritic cells with weak staining on monocytes and granulocytes.
Recently CD39 and CD73 have been found on regulatory T cells specifically the effector/memory like T cells. Functionally CD39 may allow Tregs to enter inflamed areas where high levels of ATP are present. Expression of Foxp3+ Cd4 positives cells range from 25-45% CD25+/CD39+ which his consistent with the findings of Borsellino et al.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioA1 (A1) antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This eBioA1 (A1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Please refer to the antibody vial for the recommended test volume to use for one million cells in a 100 μl total staining volume.

References

Aversa GG., M.G. Suranyi, J.A. Waugh, A.G. Bishop, B.M. Hall. 1988. Detection of a Late Lymphocyte Activation Marker by A1, a New Monoclonal Antibody. Transplant Proc. 20(1):49-52.

Aversa GG., J.A. Waugh, G.A. Bishop, B.M. Hall. 1989. Use of Monoclonal Antibodies to Study in vivo and in vitro-activated Lymphocytes. Transplant Proc. 21(1):349-50.

Stockl J., O. Majdic, G. Fischer, D. Maurer, W. Knapp. 2001. Monomorphic Molecules Function as Additional Recognition Structures on Haptenated Target Cells for HLA-A1-Restricted, Hapten-Specific CTL. J. Immunol. 167L:2724-2733

Borsellino G, Kleinewietfeld M, Di Mitri D, Sternjak A, Diamantini A, Giometto R, Hopner S, Centonze D, Bernardi G, Dell'acqua ML, Rossini PM, Battistini L, Rotzschke O, Falk K. Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression. Blood. 2007 Apr 20.

Lyck L, Dalmau I, et al. 2008. Immunohistochemical markers for quantitative studies of neurons and glia in human neocortex. J Histochem Cytochem. 56(3):201-21. (A1, IHC frozen, PubMed)

Related Products

Cat. 25-4714  Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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