Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD39 (Entpd1, NTPDase1)
Catalog Number: 25-0391
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 24DMS1
Isotype: Rat IgG2b, k

Mouse splenocytes were stained with 0.125 µg anti-mouse CD39 antibody (24DMS1) and FITC anti-mouse CD4 (GK1.5) (cat.11-0041) and then intracellularly with Alexa Fluor® 647 anti-mouse Foxp3 (FJK-16s) (cat.51-5773). The histogram (right panel) demonstrates staining of CD39 (24DMS1) on CD4+Foxp3- cells (purple) and CD4+Foxp3+ cells (blue) as gated in the dot plot (left panel).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0391	Affinity Purified anti-mouse CD39 (Entpd1, NTPDase1)	N/A	N/A	FC WB
16-0391	Functional Grade* Purified anti-mouse CD39 (Entpd1, NTPDase1)	N/A	N/A	FC WB
12-0391	Phycoerythrin (PE) anti-mouse CD39 (Entpd1, NTPDase1)	488	575	FC
25-0391	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD39 (Entpd1, NTPDase1)	488	760	FC
51-0391	Alexa Fluor® 647 anti-mouse CD39 (Entpd1, NTPDase1)	633	668	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 24DMS1 monoclonal antibody reacts with mouse CD39, also known as NTPDaseI. E-NTPDases are enzymes that convert nucleoside tri- and diphosphates (NTDPs) into nucleoside monophosphate (NMP), thereby removing toxic extracellular ATP and ADP. CD39 is the dominant member of this family in the immune system and is involved in suppression of inflammation and control of platelet activation. CD39 can impact expression of CD73, another E-NTPase. Together, these molecules influence inflammation responses. CD39 is expressed on B cells, Langerhans cells and most monocytes. In addition, CD39 is found on a subset of CD4+ T cells that are mostly CD25+FoxP3+ T reg cells. T reg cells from CD39-null mice showed impaired suppressive properties in vitro and in vivo.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 24DMS1 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This 24DMS1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39. Hyman MC, Petrovic-Djergovic D, Visovatti SH, Liao H, Yanamadala S, Bouïs D, Su EJ, Lawrence DA, Broekman MJ, Marcus AJ, Pinsky DJ. J Clin Invest. 2009 May;119(5):1136-49. (24DMS1, FC, PubMed)

Bynoe MS, Viret C. Foxp3+CD4+ T cell-mediated immunosuppression involves extracellular nucleotide catabolism.Trends Immunol. 2008 Mar;29(3):99-102.

Borsellino G, Kleinewietfeld M, Di Mitri D, Sternjak A, Diamantini A, Giometto R, Höpner S, Centonze D, Bernardi G, Dell'Acqua ML, Rossini PM, Battistini L, Rötzschke O, Falk K. Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression. Blood. 2007 Aug 15;110(4):1225-32.

Deaglio S, Dwyer KM, Gao W, Friedman D, Usheva A, Erat A, Chen JF, Enjyoji K, Linden J, Oukka M, Kuchroo VK, Strom TB, Robson SC. Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression. J Exp Med. 2007 Jun 11;204(6):1257-65.

Mizumoto N, Kumamoto T, Robson SC, Sévigny J, Matsue H, Enjyoji K, Takashima A. CD39 is the dominant Langerhans cell-associated ecto-NTPDase: modulatory roles in inflammation and immune responsiveness.Nat Med. 2002 Apr;8(4):358-65.

Sévigny J, Sundberg C, Braun N, Guckelberger O, Csizmadia E, Qawi I, Imai M, Zimmermann H, Robson SC.Differential catalytic properties and vascular topography of murine nucleoside triphosphate diphosphohydrolase 1 (NTPDase1) and NTPDase2 have implications for thromboregulation.Blood. 2002 Apr 15;99(8):2801-9.

Braun N, Sévigny J, Robson SC, Enjyoji K, Guckelberger O, Hammer K, Di Virgilio F, Zimmermann H.Assignment of ecto-nucleoside triphosphate diphosphohydrolase-1/cd39 expression to microglia and vasculature of the brain.Eur J Neurosci. 2000 Dec;12(12):4357-66.

Enjyoji K, Sévigny J, Lin Y, Frenette PS, Christie PD, Esch JS 2nd, Imai M, Edelberg JM, Rayburn H, Lech M, Beeler DL, Csizmadia E, Wagner DD, Robson SC, Rosenberg RD.Targeted disruption of cd39/ATP diphosphohydrolase results in disordered hemostasis and thromboregulation.Nat Med. 1999 Sep;5(9):1010-7.

Related Products

Cat. 11-0041  FITC anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 25-4031  Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2b Isotype Control
Cat. 51-5773  Alexa Fluor® 647 anti-mouse/rat Foxp3 (clone FJK-16s)
Cat. 24-6392  C9F/C10F serum anti-mouse CD39 (Entpd1, NTPDase1) polyclonal (clone C9F/C10F)

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