Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD28
Catalog Number: 25-0281
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7.
Clone: 37.51
Isotype: Golden Syrian Hamster IgG

Staining of BALB/c splenocytes with FITC anti-mouse CD3e (145-2C11) (cat. 11-0031) and 0.5 μg of PE-Cy7 Golden Syrian Hamster IgG Iso Cntrl (cat. 25-4914) (left) or 0.5 μg of PE-Cy7 anti-mouse CD28 (37.51) (right). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0281	PE anti-mouse CD28	488	575	FC
13-0281	Biotin anti-mouse CD28	N/A	N/A	FC
14-0281	Affinity Purified anti-mouse CD28	N/A	N/A	FA FC IHC IP
15-0281	PE-Cy5 anti-mouse CD28	488	670	FC
16-0281	Functional Grade* Purified anti-mouse CD28	N/A	N/A	FA FC
17-0281	APC anti-mouse CD28	633	660	FC
25-0281	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD28	488	760	FC
45-0281	PerCP-Cy5.5 anti-mouse CD28	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 37.51 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This 37.51 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1.0 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.

References

Nandi, D., J. A. Gross, et al. (1994). "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." J Immunol 152(7): 3361-9.
Gross, J. A., E. Callas, et al. (1992). "Identification and distribution of the costimulatory receptor CD28 in the mouse." J Immunol 149(2): 380-8.
Harding, F. A., J. G. McArthur, et al. (1992). "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." Nature 356(6370): 607-9.
Gross, J. A., T. St. John, et al. (1990). "The murine homologue of the T lymphocyte antigen CD28. Molecular cloning and cell surface expression." J Immunol 144(8): 3201-10.

Related Products

Cat. 25-0289    Phycoerythrin-Cy7 (PE-Cy7) anti-human CD28 (costimulatory) (clone CD28.2)
Cat. 25-4914    Phycoerythrin-Cy7 (PE-Cy7) Golden Syrian Hamster IgG Isotype Control (clone n/a)

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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