Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human CD25 (interleukin-2 receptor, IL-2R)
Catalog Number: 25-0259
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: BC96
Isotype: Mouse IgG1, κ
HLDA No.: V T-072

Surface staining of PHA stimulated human PBMC with FITC anti-human CD25 (BC96). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0259	May be discontinued; see superior product Alexa Fluor® 488 FITC anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	518	FC
12-0259	PE anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	575	FC
13-0259	Biotin anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	N/A	N/A	FC
14-0259	Affinity Purified anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	N/A	N/A	FC
15-0259	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	670	FC
17-0259	Allophycocyanin (APC) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	633	660	FC
25-0259	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	760	FC
45-0259	PerCP-Cy5.5 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	690	FC
53-0259	Alexa Fluor® 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	519	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The BC96 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

This product is guaranteed for 6 months upon receipt when stored properly.

Applications Tested

The BC96 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood. This can be used at 20 μl (0.25 μg) per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 25-4714    Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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