Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human CD23 (FceRII)
Catalog Number: 25-0238
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: EBVCS2
Isotype: Mouse IgG1
HLDA No.: N/A

Staining of normal human peripheral blood cells with FITC anti-human CD19 (HIB19) (cat. 11-0199) and PE-Cy7 Mouse IgG1, K Iso Cntrl (cat. 25-4714) (left) or PE-Cy7 anti-human CD23 (EBVCS2) (right). Cells in the lymphocyte and monocyte gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0238	Fluorescein (FITC) anti-human CD23 (FceRII)	488	518	FC IH/F
12-0238	Phycoerythrin (PE) anti-human CD23 (FceRII)	488	575	FC
17-0238	Allophycocyanin (APC) anti-human CD23 (FceRII)	633	660	FC
25-0238	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD23 (FceRII)	488	760	FC
14-0238	Affinity Purified anti-human CD23 (FceRII)	N/A	N/A	FC IH/F

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21, and the α subunit of CD11b and CD11c bind to CD23.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This EBVCS2 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This EBVCS2 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Bernard, A., et al. eds. (1981). Leukocyte Typing. Springer-Verlag.

Related Products

Cat. 11-0239    FITC anti-human CD23 (FceRII) (clone Tu1)
Cat. 12-0239    PE anti-human CD23 (FceRII) (clone Tu1)
Cat. 14-0239    Affinity Purified anti-human CD23 (FceRII) (clone Tu1)
Cat. 25-4714    Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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