Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD19
Catalog Number: 25-0193
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information, see PE-Cy7.
Clone: eBio1D3 (1D3)
Isotype: Rat IgG2a, κ

Staining of BALB/c splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.125 μg of PE-Cy7 Rat IgG2a Iso Cntrl (cat. 25-4321) (left) or 0.125 μg of PE-Cy7 anti-mouse CD19 (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0193	Fluorescein isothiocyanate (FITC) anti-mouse CD19	488	518	FC
12-0193	Phycoerythrin (PE) anti-mouse CD19	488	575	FC
13-0193	Biotin anti-mouse CD19	N/A	N/A	FC
14-0193	Affinity Purified anti-mouse CD19	N/A	N/A	FA FC IH/F IP
15-0193	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD19	488	670	FC
16-0193	Functional Grade* Purified anti-mouse CD19	N/A	N/A	FA FC IH/F
25-0193	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD19	488	760	FC
35-0193	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD19	488	690	FC
45-0193	PerCP-Cy5.5 anti-mouse CD19	488	690	FC
51-0193	Alexa Fluor® 647 anti-mouse CD19	633	668	FC
56-0193	Alexa Fluor® 700 anti-mouse CD19 (APC-Cy5.5 Equivalent)	633	723	FC
57-0193	Pacific Blue® anti-mouse CD19	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Cherukuri A, Cheng PC, Pierce SK. The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens. J Immunol. 2001 Jul 1;167(1):163-72.

Inabe K, Kurosaki T. Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 15;99(2):584-9.

Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol. 1996 Jan;26(1):238-42. (1D3, FA, PubMed)

Shoham T, Rajapaksa R, Boucheix C, Rubinstein E, Poe JC, Tedder TF, Levy S. The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment. J Immunol. 2003 Oct 15;171(8):4062-72.

Krop I, Shaffer AL, Fearon DT, Schlissel MS. The signaling activity of murine CD19 is regulated during cell development. J Immunol. 1996 Jul 1;157(1):48-56. (1D3, FC, FA, PubMed)

Related Products

Cat. 25-0199    Phycoerythrin-Cy7 (PE-Cy7) anti-human CD19 (clone HIB19)
Cat. 25-4321    Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2a Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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