Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD16/32 - blocks Fc binding
Catalog Number: 25-0161
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information, see PE-Cy7
Clone: 93
Isotype: Rat IgG2a, λ

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 μg PE-Cy7 anti-mouse CD16/32 (93) (colored histogram). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0161	Affinity Purified anti-mouse CD16/32 - blocks Fc binding	N/A	N/A	FA FC
16-0161	Functional Grade* Purified anti-mouse CD16/32 - blocks Fc binding	N/A	N/A	FA FC
13-0161	Biotin anti-mouse CD16/32 - blocks Fc binding	N/A	N/A	FC
11-0161	FITC anti-mouse CD16/32 - blocks Fc binding	488	518	FC
12-0161	PE anti-mouse CD16/32 - blocks Fc binding	488	575	FC
17-0161	APC anti-mouse CD16/32 - blocks Fc binding	633	660	FC
25-0161	PE-Cy7 anti-mouse CD16/32 - blocks Fc binding	488	760	FC
35-0161	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD16/32 - blocks Fc binding	488	690	FC
56-0161	Alexa Fluor® 700 anti-mouse CD16/32 - blocks Fc binding (APC-Cy5.5 Equivalent)	633	723	FC
57-0161	Pacific Blue® anti-mouse CD16/32 - blocks Fc binding	402	421	FC
45-0161	PerCP-Cy5.5 anti-mouse CD16/32 - blocks Fc binding	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fcγ III Receptor) and CD32 (Fcγ II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 93 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

The 93 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Oliver, A. M., J. C. Grimaldi, et al. (1999). "Independently ligating CD38 and Fc gammaRIIB relays a dominant negative signal to B cells." Hybridoma 18(2): 113-9.

Related Products

Cat. 25-4321    Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2a Isotype Control

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