Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human CD14
Catalog Number: 25-0149
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information, see PE-Cy7
Clone: 61D3
Isotype: Mouse IgG1, κ
HLDA No.: V MA085

Staining of normal human peripheral blood cells with PE-Cy7 Mouse IgG1, K Iso Cntrl (cat. 25-4714) (open histogram) or PE-Cy7 anti-human CD14 (61D3) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0149	Affinity Purified anti-human CD14	N/A	N/A	FA FC IHC
16-0149	Functional Grade* Purified anti-human CD14	N/A	N/A	FA FC
13-0149	Biotin anti-human CD14	N/A	N/A	FC
11-0149	FITC anti-human CD14	488	518	FC
12-0149	PE anti-human CD14	488	575	FC
17-0149	APC anti-human CD14	633	660	FC
25-0149	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD14	488	760	FC
27-0149	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD14	633	779	FC
45-0149	PerCP-Cy5.5 anti-human CD14	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 61D3 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1.0 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.
Fadok VA, Warner ML, Bratton DL, Henson PM. CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor (alpha v beta 3). J Immunol 1998 Dec 1;161(11):6250-7.

Related Products

Cat. 25-4714    Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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