Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human CD11b (Integrin alpha M, Mac-1 alpha)
Catalog Number: 25-0118
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7.
Clone: ICRF44
Isotype: Mouse IgG1, κ
HLDA No.: IV M047

Staining of normal human peripheral blood cells with PE-Cy7 Mouse IgG1, K Iso Cntrl (cat. 25-4714) (open histogram) or PE-Cy7 anti-human CD11b (ICRF44) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0118	Fluorescein isothiocyanate (FITC) anti-human CD11b (Integrin alpha M, Mac-1 alpha)	488	518	FC
12-0118	PE anti-human CD11b (Integrin alpha M, Mac-1 alpha)	488	575	FC
13-0118	Biotin anti-human CD11b (Integrin alpha M, Mac-1 alpha)	N/A	N/A	FC
14-0118	Affinity Purified anti-human CD11b (Integrin alpha M, Mac-1 alpha)	N/A	N/A	FA FC IHC
17-0118	Allophycocyanin (APC) anti-human CD11b (Integrin alpha M, Mac-1 alpha)	633	660	FC
25-0118	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD11b (Integrin alpha M, Mac-1 alpha)	488	760	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin β₂ (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This ICRF44 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This ICRF44 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1.0 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-0112    FITC anti-mouse CD11b (Integrin alpha M, Mac-1 alpha) (clone M1/70)
Cat. 25-4714    Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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