Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human CD11c
Catalog Number: 25-0116
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7.
Clone: 3.9
Isotype: Mouse IgG1, κ
HLDA No.: III NL707

Staining of normal human lysed whole blood with staining buffer (autofluorescence) (open histogram) or PE-Cy7 anti-human CD11c (3.9) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0116	Affinity Purified anti-human CD11c	N/A	N/A	FC IH/F
13-0116	Biotin anti-human CD11c	N/A	N/A	FC
11-0116	FITC anti-human CD11c	488	518	FC
12-0116	PE anti-human CD11c	488	575	FC
17-0116	Allophycocyanin (APC) anti-human CD11c	633	660	FC
25-0116	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD11c	488	760	FC
56-0116	Alexa Fluor® 700 anti-human CD11c (APC-Cy5.5 Equivalent)	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin α_X chain. CD11c non-covalently associates with β₂ integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 3.9 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This 3.9 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1 μg) per 1 million cells in 100 μl total staining volume.

References

McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 25-4714    Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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