Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human CD8a
Catalog Number: 25-0088
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: RPA-T8
Isotype: Mouse IgG1, κ
HLDA No.: IV T171

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or PE-Cy7 RPA-T8 (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0088	Affinity Purified anti-human CD8 (CD8a; alpha chain)	N/A	N/A	FC IH/F
11-0088	FITC anti-human CD8 (CD8a; alpha chain)	488	518	FC
12-0088	PE anti-human CD8 (CD8a; alpha chain)	488	575	FC
15-0088	PE-Cy5 anti-human CD8 (CD8a; alpha chain)	488	670	FC
17-0088	APC anti-human CD8 (CD8a; alpha chain)	633	660	FC
25-0088	PE-Cy7 anti-human CD8 (CD8a; alpha chain)	488	760	FC
45-0088	PerCP-Cy5.5 anti-human CD8 (CD8a; alpha chain) (PE-Cy5.5 replacement)	488	690	FC
93-0088	eFluor^TM 605^NC anti-human CD8 (CD8a; alpha chain)	350-500	605	FC
94-0088	eFluor^TM 625^NC anti-human CD8 (CD8a; alpha chain)	350-500	625	FC
95-0088	eFluor^TM 650^NC anti-human CD8 (CD8a; alpha chain)	350-500	650	FC
47-0088	Allophycocyanin-eFluor^TM 780 anti-human CD8 (CD8a; alpha chain) (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0088	eFluor^TM 450 anti-human CD8 (CD8a; alpha chain) (Pacific Blue® replacement)	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RPA-T8 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.

Related Products

Cat. 11-0089  FITC anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 12-0089  PE anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 13-0089  Biotin anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 14-0089  Affinity Purified anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 16-0089  Functional Grade Purified anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 25-4714  Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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