Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human CD5
Catalog Number: 25-0059
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7.
Clone: UCHT2
Isotype: Mouse IgG1, κ
HLDA No.: III 518

Staining of normal human peripheral blood cells with FITC anti-human CD19 (HIB19) (cat. 11-0199) and PE-Cy7 Mouse IgG1, K Iso Cntrl (cat. 25-4714) (left) or PE-Cy7 anti-human CD5 (UCHT2) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0059	FITC anti-human CD5	488	518	FC
12-0059	PE anti-human CD5	488	575	FC
13-0059	Biotin anti-human CD5	N/A	N/A	FC
14-0059	Affinity Purified anti-human CD5	N/A	N/A	FC IHC WB
25-0059	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD5	488	760	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This UCHT2 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

This UCHT2 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 25-4714    Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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