Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD5 (Ly-1)
Catalog Number: 25-0051
Sizes: 50 ug
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 53-7.3
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.125 μg of PE-Cy7 Rat IgG2a Iso Cntrl (cat. 25-4321) (left) or 0.125 μg of PE-Cy7 anti-mouse CD5 (53-7.3) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0051	Affinity Purified anti-mouse CD5 (Ly-1)	N/A	N/A	FC IHC IP
13-0051	Biotin anti-mouse CD5 (Ly-1)	N/A	N/A	FC
11-0051	FITC anti-mouse CD5 (Ly-1)	488	518	FC
12-0051	PE anti-mouse CD5 (Ly-1)	488	575	FC
17-0051	Allophycocyanin (APC) anti-mouse CD5 (Ly-1)	633	660	FC
25-0051	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD5 (Ly-1)	488	760	FC
45-0051	PerCP-Cy5.5 anti-mouse CD5 (Ly-1) (PE-Cy5.5 replacement)	488	690	FC
48-0051	eFluor^TM 450 anti-mouse CD5 (Ly-1)	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 53-7.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ledbetter, J. A. and L. A. Herzenberg. 1979. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47: 63-90.
Ledbetter, J. A., R. V. Rouse, et al. 1980. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med 152(2): 280-95.

Related Products

Cat. 11-0050  FITC anti-rat CD5 (clone HIS47)
Cat. 12-0050  PE anti-rat CD5 (clone HIS47)
Cat. 14-0050  Affinity Purified anti-rat CD5 (clone HIS47)
Cat. 15-0050  Phycoerythrin-Cy5 (PE-Cy5) anti-rat CD5 (clone HIS47)
Cat. 17-0050  Allophycocyanin (APC) anti-rat CD5 (clone HIS47)
Cat. 11-0059  FITC anti-human CD5 (clone UCHT2)
Cat. 12-0059  PE anti-human CD5 (clone UCHT2)
Cat. 13-0059  Biotin anti-human CD5 (clone UCHT2)
Cat. 14-0059  Affinity Purified anti-human CD5 (clone UCHT2)
Cat. 25-0059  Phycoerythrin-Cy7 (PE-Cy7) anti-human CD5 (clone UCHT2)
Cat. 25-4321  Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2a Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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