Anti-Mouse CD4 PE-Cy7

Also known as: L3T4, Ly-4

RUO: For Research Use Only

mouse CD4 antibody, Phycoerythrin-Cy7 (PE-Cy7) conjugate

Staining of mouse splenocytes with Anti-Mouse CD4 FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Contents: Anti-Mouse CD4 PE-Cy7
Catalog Number: 25-0042
Concentration: 0.2 mg/ml
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN₃, protein stabilizer
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: RM4-5
Host/Isotype: Rat IgG2a, κ

Product Options
Cat. No.	Size	Price	Add Qty to Cart
25-0042-81	50 ug	To view relevant pricing, please select your country and resume browsing.
25-0042-82	100 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-0041	Anti-Mouse CD4 FITC (GK1.5)
12-0041	Anti-Mouse CD4 PE (GK1.5)
13-0041	Anti-Mouse CD4 Biotin (GK1.5)
14-0041	Anti-Mouse CD4 Purified (GK1.5)
15-0041	Anti-Mouse CD4 PE-Cy5 (GK1.5)
16-0041	Anti-Mouse CD4 Functional Grade Purified (GK1.5)
17-0041	Anti-Mouse CD4 APC (GK1.5)
19-0041	Anti-Mouse CD4 Cy5 (GK1.5)
25-0041	Anti-Mouse CD4 PE-Cy7 (GK1.5)
25-4321	Rat IgG2a K Isotype Control PE-Cy7

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-0042	Anti-Mouse CD4 FITC	488	518	FC	RUO
12-0042	Anti-Mouse CD4 PE	488	575	FC	RUO
13-0042	Anti-Mouse CD4 Biotin	N/A	N/A	FC	RUO
14-0042	Anti-Mouse CD4 Purified	N/A	N/A	FA FC IHC (F)	RUO
15-0042	Anti-Mouse CD4 PE-Cy5	488	670	FC	RUO
16-0042	Anti-Mouse CD4 Functional Grade* Purified	N/A	N/A	FC	RUO
17-0042	Anti-Mouse CD4 APC	633	660	FC	RUO
25-0042	Anti-Mouse CD4 PE-Cy7	488	760	FC	RUO
45-0042	Anti-Mouse CD4 PerCP-Cy5.5	488	690	FC	RUO
46-0042	Anti-Mouse CD4 PerCP-eFluor® 710	488	710	FC	RUO
47-0042	Anti-Mouse CD4 APC-eFluor® 780	633	780	FC	RUO
48-0042	Anti-Mouse CD4 eFluor® 450 (Pacific Blue® replacement)	405	450	FC	RUO
56-0042	Anti-Mouse CD4 Alexa Fluor® 700	633	723	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RM4-5 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

This product is guaranteed for 6 months upon receipt when stored properly.

Applications Tested

The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Bliss SK, Bliss SP, Beiting DP, Alcaraz A, Appleton JA. IL-10 regulates movement of intestinally derived CD4+ T cells to the liver. J Immunol. 2007 Jun 15;178(12):7974-83. (RM4-5, FC, PubMed)

Rubinstein MP, Kovar M, Purton JF, Cho JH, Boyman O, Surh CD, Sprent J. Converting IL-15 to a superagonist by binding to soluble IL-15R{alpha}. Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9166-71. (RM4-5, FC, PubMed)

Irie J, Wu Y, Wicker LS, Rainbow D, Nalesnik MA, Hirsch R, Peterson LB, Leung PS, Cheng C, Mackay IR, Gershwin ME, Ridgway WM. NOD.c3c4 congenic mice develop autoimmune biliary disease that serologically and pathogenetically models human primary biliary cirrhosis. J Exp Med. 2006 May 15;203(5):1209-19. (RM4-5, IHC frozen, PubMed)

Andres PG, Beck PL, Mizoguchi E, Mizoguchi A, Bhan AK, Dawson T, Kuziel WA, Maeda N, MacDermott RP, Podolsky DK, Reinecker HC. Mice with a selective deletion of the CC chemokine receptors 5 or 2 are protected from dextran sodium sulfate-mediated colitis: lack of CC chemokine receptor 5 expression results in a NK1.1+ lymphocyte-associated Th2-type immune response in the intestine. J Immunol. 2000 Jun 15;164(12):6303-12. (RM4-5, IHC frozen)

Okumura, K. 2000.Personal communication.

Shi Y, Kaliyaperumal A, Lu L, Southwood S, Sette A, Michaels MA, Datta SK. Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain. J Exp Med. 1998 Feb 2;187(3):367-78. (RM4-5, FA)

Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83.

Legal

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS.

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