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Anti-Mouse CD4 PE-Cy7

Also known as: L3T4, Ly-4

RUO: For Research Use Only
mouse CD4 antibody, Phycoerythrin-Cy7 (PE-Cy7) conjugatemouse CD4 antibody, Phycoerythrin-Cy7 (PE-Cy7) conjugateStaining of mouse splenocytes with Anti-Mouse CD4 FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse CD4 PE-Cy7
Catalog Number: 25-0042
Concentration: 0.2 mg/ml
Formulation: Phosphate buffered saline pH 7.2, less than or equal to 0.09% NaN3, protein stabilizer
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: RM4-5
Host/Isotype: Rat IgG2a, κ
 
Product Options
Cat. No. Size Price Add Qty to Cart
25-0042-81 50 ug
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25-0042-82 100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
11-0041 Anti-Mouse CD4 FITC (GK1.5)
12-0041 Anti-Mouse CD4 PE (GK1.5)
13-0041 Anti-Mouse CD4 Biotin (GK1.5)
14-0041 Anti-Mouse CD4 Purified (GK1.5)
15-0041 Anti-Mouse CD4 PE-Cy5 (GK1.5)
16-0041 Anti-Mouse CD4 Functional Grade Purified (GK1.5)
17-0041 Anti-Mouse CD4 APC (GK1.5)
19-0041 Anti-Mouse CD4 Cy5 (GK1.5)
25-0041 Anti-Mouse CD4 PE-Cy7 (GK1.5)
25-4321 Rat IgG2a K Isotype Control PE-Cy7
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
11-0042 Anti-Mouse CD4 FITC 488 518 FC RUO
12-0042 Anti-Mouse CD4 PE 488 575 FC RUO
13-0042 Anti-Mouse CD4 Biotin N/A N/A FC RUO
14-0042 Anti-Mouse CD4 Purified N/A N/A FA FC IHC (F) RUO
15-0042 Anti-Mouse CD4 PE-Cy5 488 670 FC RUO
16-0042 Anti-Mouse CD4 Functional Grade* Purified N/A N/A FC RUO
17-0042 Anti-Mouse CD4 APC 633 660 FC RUO
25-0042 Anti-Mouse CD4 PE-Cy7 488 760 FC RUO
45-0042 Anti-Mouse CD4 PerCP-Cy5.5 488 690 FC RUO
46-0042 Anti-Mouse CD4 PerCP-eFluor® 710 488 710 FC RUO
47-0042 Anti-Mouse CD4 APC-eFluor® 780 633 780 FC RUO
48-0042 Anti-Mouse CD4 eFluor® 450 (Pacific Blue® replacement) 405 450 FC RUO
56-0042 Anti-Mouse CD4 Alexa Fluor® 700 633 723 FC RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The RM4-5 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

This product is guaranteed for 6 months upon receipt when stored properly.
Applications Tested
The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Bliss SK, Bliss SP, Beiting DP, Alcaraz A, Appleton JA. IL-10 regulates movement of intestinally derived CD4+ T cells to the liver. J Immunol. 2007 Jun 15;178(12):7974-83. (RM4-5, FC, PubMed)

Rubinstein MP, Kovar M, Purton JF, Cho JH, Boyman O, Surh CD, Sprent J. Converting IL-15 to a superagonist by binding to soluble IL-15R{alpha}. Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9166-71. (RM4-5, FC, PubMed)

Irie J, Wu Y, Wicker LS, Rainbow D, Nalesnik MA, Hirsch R, Peterson LB, Leung PS, Cheng C, Mackay IR, Gershwin ME, Ridgway WM. NOD.c3c4 congenic mice develop autoimmune biliary disease that serologically and pathogenetically models human primary biliary cirrhosis. J Exp Med. 2006 May 15;203(5):1209-19. (RM4-5, IHC frozen, PubMed)

Andres PG, Beck PL, Mizoguchi E, Mizoguchi A, Bhan AK, Dawson T, Kuziel WA, Maeda N, MacDermott RP, Podolsky DK, Reinecker HC. Mice with a selective deletion of the CC chemokine receptors 5 or 2 are protected from dextran sodium sulfate-mediated colitis: lack of CC chemokine receptor 5 expression results in a NK1.1+ lymphocyte-associated Th2-type immune response in the intestine. J Immunol. 2000 Jun 15;164(12):6303-12. (RM4-5, IHC frozen)

Okumura, K. 2000.Personal communication.

Shi Y, Kaliyaperumal A, Lu L, Southwood S, Sette A, Michaels MA, Datta SK. Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain. J Exp Med. 1998 Feb 2;187(3):367-78. (RM4-5, FA)

Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83.

Legal
FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.

COMMERCIAL USE shall include:
1. sale, lease, license or other transfer of the material or any material derived or produced from it;
2. sale, lease, license or other grant of rights to use this Material or any material derived or produced from it;
2. use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.

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