PE-Cy7 anti-mouse CD4 (L3T4) antibody: Cy7PE conjugate

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Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD4 (L3T4)
Catalog Number: 25-0041
Formulation: Phosphate buffer saline pH 7.2, less than or equal to 0.09% NaN₃,protein stabilizer
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7
Clone: GK1.5
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
25-0041-81	50 ug	$120
25-0041-82	100 ug	$195

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.125 μg of PE-Cy7 Rat IgG2b Iso Cntrl (cat. 25-4331) (left) or 0.125 μg of PE-Cy7 anti-mouse CD4 (GK1.5) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0041	FITC anti-mouse CD4 (L3T4)	488	518	FC
12-0041	PE anti-mouse CD4 (L3T4)	488	575	FC
13-0041	Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC
14-0041	Affinity Purified anti-mouse CD4 (L3T4)	N/A	N/A	FC IH/F IP
15-0041	PE-Cy5 anti-mouse CD4 (L3T4)	488	670	FC
16-0041	Functional Grade* Purified anti-mouse CD4 (L3T4)	N/A	N/A	FC
17-0041	APC anti-mouse CD4 (L3T4)	633	660	FC
19-0041	Cy5 anti-mouse CD4 (L3T4)	633	670	FC
25-0041	PE-Cy7 anti-mouse CD4 (L3T4)	488	760	FC
36-0041	Functional Grade* Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC IH/F IP
51-0041	Alexa Fluor® 647 anti-mouse CD4 (L3T4)	633	668	FC
53-0041	Alexa Fluor® 488 anti-mouse CD4 (L3T4)	488	519	FC
56-0041	Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent)	633	723	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The GK1.5 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

The GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Dialynas, D. P., Z. S. Quan, et al. (1983). "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." J Immunol 131(5): 2445-51.
Wilde, D. B., P. Marrack, et al. (1983). "Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen- specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines." J Immunol 131(5): 2178-83.

Related Products

Cat. 11-0042    FITC anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 12-0042    PE anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 17-0042    APC anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 25-0042    PE-Cy7 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 35-0042    PE-Cy5.5 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 12-0043    Phycoerythrin (PE) anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 25-4331    Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2b Isotype Control

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