Product Information

Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-human CD3
Catalog Number: 25-0038
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7.
Clone: UCHT1
Isotype: Mouse IgG1, κ
HLDA No.: III 471

Surface staining of normal human peripheral blood cells with anti-human CD3 (UCHT1) PE-Cy5 (left), and APC (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0038	FITC anti-human CD3	488	518	FC
12-0038	PE anti-human CD3	488	575	FC
13-0038	Biotin anti-human CD3	N/A	N/A	FC
14-0038	Affinity Purified anti-human CD3	N/A	N/A	FA FC IH/F
15-0038	PE-Cy5 anti-human CD3	488	670	FC
16-0038	Functional Grade* Purified anti-human CD3	N/A	N/A	FA FC
17-0038	APC anti-human CD3	633	660	FC
25-0038	PE-Cy7 anti-human CD3	488	760	FC
27-0038	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD3 (APC-Cy7 Equivalent)	633	779	FC
56-0038	Alexa Fluor® 700 anti-human CD3 (APC-Cy5.5 Equivalent)	633	723	FC
57-0038	Pacific Blue® anti-human CD3	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This UCHT1 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

The UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-0037    FITC anti-human CD3 (clone OKT3)
Cat. 12-0037    PE anti-human CD3 (clone OKT3)
Cat. 14-0037    Affinity Purified anti-human CD3 (clone OKT3)
Cat. 16-0037    Functional Grade Purified anti-human CD3 (clone OKT3)
Cat. 11-0039    FITC anti-human CD3 (clone HIT3a)
Cat. 12-0039    PE anti-human CD3 (clone HIT3a)
Cat. 14-0039    Affinity Purified anti-human CD3 (clone HIT3a)
Cat. 16-0039    Functional Grade Purified anti-human CD3 (clone HIT3a)
Cat. 25-4714    Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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