PE-Cy7 anti-mouse CD3e antibody: Cy7PE conjugated anti-mouse CD3 mAb

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Contents: Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD3e
Catalog Number: 25-0031
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage, handling & experimental procedures.
This product has been improved, resulting in brighter staining with less compensation required. For more information see PE-Cy7
Clone: 145-2C11
Isotype: Armenian Hamster IgG

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
25-0031-81	50 ug	$120
25-0031-82	100 ug	$195

*International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with PE anti-mo/hu B220 (RA3-6B2) (cat. 12-0452) and 0.5 μg of PE-Cy7 Armenian Hamster IgG Iso Cntrl (cat. 25-4888) (left) or 0.5 μg of PE-Cy7 anti-mouse CD3e (145-2C11) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0031	FITC Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	518	FC
12-0031	PE Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	575	FC
13-0031	Biotin Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FC
14-0031	Affinity Purified Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC IH/F IP WB
15-0031	PE-Cy5 Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	670	FC
16-0031	Functional Grade* Purified Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC
17-0031	APC Hamster anti-mouse CD3 (epsilon subunit; CD3e)	633	660	FC
25-0031	PE-Cy7 Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	760	FC
35-0031	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD3 (epsilon subunit; CD3e)	488	690	FC
36-0031	Functional Grade* Biotin anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC IH/F IP WB
45-0031	PerCP-Cy5.5 anti-mouse CD3 (epsilon subunit; CD3e)	488	690	FC
53-0031	Alexa Fluor® 488 anti-mouse CD3 (epsilon subunit; CD3e)	488	519	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 145-2C11 antibody has been reported for use in flow cytometric analysis.

This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

Applications Tested

The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Leo, O., M. Foo, et al. (1987). "Identification of a monoclonal antibody specific for a murine T3 polypeptide." Proc Natl Acad Sci U S A 84(5): 1374-8.
Samelson, L. E., J. J. O'Shea, et al. (1987). "T cell antigen receptor phosphorylation induced by an anti-receptor antibody." J Immunol 139(8): 2708-14.

Related Products

Cat. 25-4888    Phycoerythrin-Cy7 (PE-Cy7) Armenian Hamster IgG Isotype Control (clone eBio299Arm)

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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