Product Information

Contents: Rabbit serum anti-mouse Toll-like receptor 4 (CD284, TLR4, TLR-4, Western Blotting Antibody)
Catalog Number: 24-9004
Sizes: 25 ul
Formulation: serum, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Clone: polyclonal
Isotype: Rabbit IgG

Immunoblotting of thioglycolated mouse PEC cell lysate from 10⁵ cells; probed with 1:1000 dilution of rabbit anti-mouse TLR4 serum (Lane A) or 1:1000 dilution of rabbit pre-bleed serum (Lane B) and revealed with HRP anti-rabbit IgG.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
24-9004	Rabbit serum anti-mouse Toll-like receptor 4 (CD284, TLR4, TLR-4, Western Blotting Antibody)	N/A	N/A	WB

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Description

The mouse TLR4 polyclonal antibody is generated against a bacterially expressed fragment of the mouse TLR4 extracellular region. So far, at least twelve members of the Toll family have been identified. This family of type I transmembrane protein is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4, which is approximately 100 kDa, is expressed by myeloid lineage cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested

This polyclonal antibody has been tested by immunoblotting (WB) of thioglycolated mouse PEC cell lysate (1:1000). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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