Product Information

Contents: Cocktail of FITC & APC Mouse IgG1 Isotype Control (For use with cat. 22-0425, 88-8999)
Catalog Number: 22-1714
Sizes: 10 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Isotype: Mouse IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
22-1714	Cocktail of FITC & APC Mouse IgG1 Isotype Control (For use with cat# 22-0425, 88-8999)	488(FITC)/488(PE)	518(FITC)/575(PE)	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

This cocktaill of FITC & APC Mouse IgG1 Isotype Control should be used with the cocktail of FITC anti-human CD4 (RPA-T4) and APC anti-human CD25 (BC96) (cat. 22-0425) and the Human Regulatory T cell Staining Kit (PE Foxp3 PCH101, FITC CD4, APC CD25) (cat. 88-8999).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This cocktail of FITC & APC mIgG1 Isotype Control has been reported for use in flow cytometric analysis.

Applications Tested

This can be used at 20 μl (1 μg FITC and 0.125 μg APC) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

Related Products

Cat. 22-0425 Cocktail of FITC anti-human CD4 (RPA-T4) and APC anti-human CD25 (BC96) (clone RPA-T4 (CD4) and BC96 (CD25))
Cat. 88-8999 Human Regulatory T cell Staining Kit (w/ PE Foxp3 PCH101, FITC CD4, APC CD25; Treg Kit) (clone PCH101)