Product Information

Contents: Cocktail of FITC anti-human CD4 (RPA-T4) and APC anti-human CD25 (BC96)
Catalog Number: 22-0425
Sizes: 25 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RPA-T4 (CD4) and BC96 (CD25)

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
22-0425	Cocktail of FITC anti-human CD4 (RPA-T4) and APC anti-human CD25 (BC96)	488(FITC)/488(PE)	518(FITC)/575(PE)	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

This cocktail of FITC anti-human CD4 (RPA-T4) and APC anti-human CD25 (BC96) can be used for the flow cytometric detection of human Regulatory-T cells. It has become well-established that the co-expression of CD4 and CD25 together with Foxp3 is a reliable marker for the identification of these T cells, which are of critical importance to the immune system.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RPA-T4 (CD4) and BC96 (CD25) antibodies have been reported for use in flow cytometric analysis.

Applications Tested

This RPA-T4 (CD4) and BC96 (CD25) antibody cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood mononuclear cells. This can be used at 20 μl (1μg of RPA-T4 and 0.125μg BC96) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

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