Product Information

Contents: Cocktail of Fluorescein isothiocyanate (FITC) anti-human CD3 (UCHT1) and Phycoerythrin (PE) anti-human CD4 (RPA-T4)
Catalog Number: 22-0304
Sizes: 50 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
22-0304	Cocktail of FITC anti-human CD3 (UCHT1) and PE anti-human CD4 (RPA-T4)	488(FITC)/488(PE)	518(FITC)/575(PE)	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

This product consists of two fluorochrome-conjugated monoclonal antibodies to distinguish human peripheral CD3⁺ and CD4⁺ T cells. The UCHT1 monoclonal antibody (mouse IgG1, κ, HLDA #III471) reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The RPA-T4 monoclonal antibody (mouse IgG1, κ, HLDA #IVT114) reacts with the human CD4 molecule, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This product is formulated for use in two-color analysis by flow cytometric analysis.

Applications Tested

Cocktail: FITC anti-hCD3 (UCHT1) and PE anti-hCD4 (RPA-T4) has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.