Cy5 anti-mouse Ly-6G (Gr-1, Gr1)

RUO: For Research Use Only

Staining of mouse bone marrow cells with FITC anti-CD45R/B220 (cat.11-0452) alone (left) or with 0.06 µg of Cy5 RB6-8C5(right). Total viable cells were used for analysis.

Contents: Cy5 anti-mouse Ly-6G (Gr-1, Gr1)
Catalog Number: 19-5931
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Clone: RB6-8C5
Host/Isotype: Rat IgG2b, κ
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
19-5931-81	50 ug	To view relevant pricing, please select your country and resume browsing.
19-5931-82	100 ug
19-5931-83	200 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-5931	FITC anti-mouse Ly-6G (Gr-1, Gr1)	488	518	FC	RUO
12-5931	PE anti-mouse Ly-6G (Gr-1, Gr1)	488	575	FC	RUO
13-5931	Biotin anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FC	RUO
14-5931	Affinity Purified anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FA FC IH/F IHC(Paraffin) IP WB	RUO
15-5931	PE-Cy5 anti-mouse Ly-6G (Gr-1, Gr1)	488	670	FC	RUO
16-5931	Functional Grade* Purified anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FA FC	RUO
17-5931	Allophycocyanin (APC) anti-mouse Ly-6G (Gr-1, Gr1)	633	660	FC	RUO
19-5931	Cy5 anti-mouse Ly-6G (Gr-1, Gr1)	633	670	FC	RUO
25-5931	PE-Cy7 anti-mouse Ly-6G (Gr-1, Gr1)	488	760	FC	RUO
35-5931	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse Ly-6G (Gr-1, Gr1)	488	690	FC	RUO
36-5931	Functional Grade* Biotin anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FC	RUO
45-5931	PerCP-Cy5.5 anti-mouse Ly-6G (Gr-1, Gr1) (PE-Cy5.5 replacement)	488	690	FC	RUO
47-5931	Allophycocyanin-eFluor® 780 anti-mouse Ly-6G (Gr-1, Gr1) (APC-Alexa Fluor® 750 replacement)	633	780	FC	RUO
48-5931	eFluor® 450 anti-mouse Ly-6G (Gr-1, Gr1) (Pacific Blue® Replacement)	405	450	FC	RUO
51-5931	Alexa Fluor® 647 anti-mouse Ly-6G (Gr-1, Gr1)	633	668	FC	RUO
53-5931	Alexa Fluor® 488 anti-mouse Ly-6G (Gr-1, Gr1)	488	519	FC	RUO
56-5931	Alexa Fluor® 700 anti-mouse Ly-6G (Gr-1, Gr1) (APC-Cy5.5 Equivalent)	633	723	FC	RUO
94-5931	eFluor® 625NC anti-mouse GR-1(Gr-1, Gr1)	350-500	625	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G and Ly-6C, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RB6-8C5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sasmono RT, Ehrnsperger A, Cronau SL, Ravasi T, Kandane R, Hickey MJ, Cook AD, Himes SR, Hamilton JA, Hume DA. Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1. J Leukoc Biol. 2007 Jul;82(1):111-23. (RB6-8C5, Cross-reactivity to Ly-6G and Ly-6C, Pubmed)

Huang B, Zhao J, Shen S, Li H, He KL, Shen GX, Mayer L, Unkeless J, Li D, Yuan Y, Zhang GM, Xiong H, Feng ZH. Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Res. 2007 May 1;67(9):4346-52. (RB6-8C5, in vivo depletion, PubMed)

Fan J, Li Y, Vodovotz Y, Billiar TR, Wilson MA. Hemorrhagic shock-activated neutrophils augment TLR4 signaling-induced TLR2 upregulation in alveolar macrophages: role in hemorrhage-primed lung inflammation. Am J Physiol Lung Cell Mol Physiol. 2006 Apr;290(4):L738-L746. (RB6-8C5, in vivo depletion, PubMed)

Alvarez MJ, Prada F, Salvatierra E, Bravo AI, Lutzky VP, Carbone C, Pitossi FJ, Chuluyan HE, Podhajcer OL. Secreted protein acidic and rich in cysteine produced by human melanoma cells modulates polymorphonuclear leukocyte recruitment and antitumor cytotoxic capacity. Cancer Res. 2005 Jun 15;65(12):5123-32. (RB6-8C5, IHC paraffin, Pubmed)

Brown CR, Blaho VA, Loiacono CM. Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2. J Immunol. 2003 Jul 15;171(2):893-901. (RB6-8C5, IHC paraffin, Pubmed)

Miura M, Fu X, Zhang QW, Remick DG, Fairchild RL. Neutralization of Gro alpha and macrophage inflammatory protein-2 attenuates renal ischemia/reperfusion injury. Am J Pathol. 2001 Dec;159(6):2137-45. (RB6-8C5, IHC frozen, Pubmed)

Dunussi-Joannopoulos K, Runyon K, Erickson J, Schaub RG, Hawley RG, Leonard JP. Vaccines with interleukin-12-transduced acute myeloid leukemia cells elicit very potent therapeutic and long-lasting protective immunity. Blood. 1999 Dec 15;94(12):4263-73. (RB6-8C5, IHC frozen, Pubmed)

Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993 Sep 1;151(5):2399-408. (RB6-8C5, Cross-reactivity to Ly-6G and Ly-6C, Pubmed)

Hestdal K, Ruscetti FW, Ihle JN, Jacobsen SE, Dubois CM, Kopp WC, Longo DL, Keller JR. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J Immunol. 1991 Jul 1;147(1):22-8.

Legal

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS.

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